从印度南部维洛尔的散发性肝炎病例和污水样本中鉴定戊型肝炎病毒。
Characterization of hepatitis E virus from sporadic hepatitis cases and sewage samples from Vellore, south India.
机构信息
Wellcome Trust Research Laboratory, Division of Gastrointestinal Sciences, Christian Medical College, Vellore 632004, India.
出版信息
Trans R Soc Trop Med Hyg. 2013 Jun;107(6):363-7. doi: 10.1093/trstmh/trt030.
BACKGROUND
Hepatitis E virus (HEV) is endemic in India and causes epidemics and sporadic cases. However, the exact transmission route for sporadic hepatitis E remains unclear. This study investigated HEV in sporadic hepatitis cases and sewage samples, as sewage is the major source of contamination of water in developing countries.
METHODS
Monthly sampling and testing for HEV in sewage samples from Vellore, India was carried out for 1 year (November 2009-October 2010) and plasma and/or fecal samples from sporadic hepatitis cases presenting to a hospital in Vellore during 2006-2010 were tested for HEV RNA. A total of 144 raw sewage samples and 94 samples from sporadic hepatitis cases were tested for HEV RNA using RT-PCR.
RESULTS
The prevalence of HEV RNA in sewage and sporadic cases was 55.6% and 9.6%, respectively. HEV strains isolated from sewage showed 94-100% nucleotide sequence similarity with the HEV strains isolated from the sporadic hepatitis cases. HEV RNA in sewage was identified more often during the summer (81.2%) than the monsoon season (14.5%) (p < 0.001).
CONCLUSION
This study indicates that sewage may be a source of contamination for sporadic hepatitis and also underscores the need for preventive measures to protect drinking water from sewage contamination, particularly in the summer. GENBANK ACCESSION NUMBERS: HEV strains isolated from this study were deposited in GenBank under accession numbers JF972766-JF972773, JN705651-JN705659 and JN705660-JN705662.
背景
戊型肝炎病毒（HEV）在印度流行，并引发暴发和散发病例。然而，散发性戊型肝炎的确切传播途径仍不清楚。本研究调查了散发性肝炎病例和污水样本中的 HEV，因为污水是发展中国家水污染的主要来源。
方法
对印度维洛尔的污水样本进行每月采样和检测，时间为 2009 年 11 月至 2010 年 10 月；对 2006 年至 2010 年期间在维洛尔一家医院就诊的散发性肝炎病例的血浆和/或粪便样本进行 HEV RNA 检测。共检测了 144 份原始污水样本和 94 份散发性肝炎病例样本，采用 RT-PCR 检测 HEV RNA。
结果
污水和散发性病例中 HEV RNA 的流行率分别为 55.6%和 9.6%。从污水中分离出的 HEV 株与从散发性肝炎病例中分离出的 HEV 株的核苷酸序列相似度为 94%-100%。污水中 HEV RNA 的检出率在夏季（81.2%）高于季风期（14.5%）（p<0.001）。
结论
本研究表明，污水可能是散发性肝炎的污染源，并强调需要采取预防措施，保护饮用水免受污水污染，特别是在夏季。基因库 accession 编号：本研究分离的 HEV 株已存入基因库，登录号为 JF972766-JF972773、JN705651-JN705659 和 JN705660-JN705662。
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