Department of Immunobiology, University of Arizona, Tucson, AZ 85721, USA.
BIO5 Institute, University of Arizona, Tucson, AZ 85721, USA.
J Gen Virol. 2013 Aug;94(Pt 8):1865-1869. doi: 10.1099/vir.0.053694-0. Epub 2013 May 15.
Cathepsin L (CatL) and cathepsin B (CatB) are lysosomal proteases that many viruses utilize for capsid disassembly. We tested whether CatL and CatB are required for infection by human papillomavirus type 16 (HPV16). CatL- and CatB-deficient mouse embryonic fibroblasts had higher levels of infection when compared with wild-type cells. Similar results were obtained in HaCaT keratinocytes treated with CatL- or CatB-specific small interfering RNA. Thus, CatL and CatB are not required for HPV16 infection but instead appear to restrict infection.
组织蛋白酶 L（CatL）和组织蛋白酶 B（CatB）是溶酶体蛋白酶，许多病毒利用它们进行衣壳解体。我们测试了 CatL 和 CatB 是否是感染人乳头瘤病毒 16 型（HPV16）所必需的。与野生型细胞相比，CatL 和 CatB 缺陷型小鼠胚胎成纤维细胞的感染水平更高。用 CatL 或 CatB 特异性小干扰 RNA 处理的 HaCaT 角质形成细胞也得到了类似的结果。因此，CatL 和 CatB 不是 HPV16 感染所必需的，而是似乎限制了感染。
