Gunawardana V K
Department of Preclinical Studies, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Sri Lanka.
Tissue Cell. 1990;22(2):113-21. doi: 10.1016/0040-8166(90)90015-2.
Alkaline phosphatase activity in the intertubular tissue of the testes of the domestic fowl was examined using an ultracytochemical technique based on the lead capture method. In the interstitial tissue, the Leydig cells, transitional cells and the fibroblasts displayed enzyme activity on their cell membranes. Vacuoles located in the transitional cells were lined by reaction products of enzyme activity, whereas the vacuoles representing extracted lipid droplets and present mainly in the Leydig cells were free of enzyme activity. In the peritubular tissue the cell processes of fibroblasts showed enzyme activity on the cell membranes and in pinocytotic vesicles. Cell processes lying adjacent to blood vessels showed pronounced activity. In the blood vessel itself some activity was present in the basement membrane and the endothelium. The surface of the red blood cell showed moderate activity. The possible role of alkaline phosphatase in the transfer of hormone from the Leydig cells to the seminiferous tubules and from the seminiferous tubules to the interstitium is discussed. The myoid cells and their processes were devoid of enzyme activity.
采用基于铅捕获法的超微细胞化学技术，对家鸡睾丸曲管间组织中的碱性磷酸酶活性进行了检测。在间质组织中，睾丸间质细胞、过渡细胞和成纤维细胞在其细胞膜上呈现酶活性。位于过渡细胞中的液泡被酶活性反应产物所包绕，而主要存在于睾丸间质细胞中代表脂质小滴提取物的液泡则无酶活性。在曲管周围组织中，成纤维细胞的细胞突起在细胞膜和胞饮小泡中呈现酶活性。与血管相邻的细胞突起显示出明显的活性。在血管本身，基底膜和内皮中有一些活性。红细胞表面显示出中度活性。文中讨论了碱性磷酸酶在激素从睾丸间质细胞向生精小管以及从生精小管向间质转移过程中的可能作用。肌样细胞及其突起无酶活性。
