[嗜热栖热菌HB-27酪氨酰-tRNA合成酶的分离]
[Isolation of tyrosyl-tRNA-synthetase from Thermus thermophilus HB-27].
作者信息
Iaremchuk A D, Tukalo M A, Egorova S P, Konovalenko A V, Matsuka G Kh
出版信息
Ukr Biokhim Zh (1978). 1990 Mar-Apr;62(2):97-9.
PMID:2368192
Abstract
A method for isolating tyrosyl-tRNA synthetase from Thermus thermophilus is described, including ammonium sulfate fractionation, chromatography on DEAE-sepharose, hydroxyapatite, heparin-sepharose and hydrophobic chromatography on Toyopearl HW-65. The yield of the purified enzyme was 1.6 mg per 1 kg of T. thermophilus cells. The enzyme is a dimer protein of the alpha 2 type with molecular weight of 100 kDa.
摘要
描述了一种从嗜热栖热菌中分离酪氨酰 - tRNA合成酶的方法，包括硫酸铵分级分离、DEAE - 琼脂糖柱层析、羟基磷灰石柱层析、肝素 - 琼脂糖柱层析以及在Toyopearl HW - 65上的疏水层析。每1千克嗜热栖热菌细胞中纯化酶的产量为1.6毫克。该酶是一种α2型二聚体蛋白，分子量为100 kDa。
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