从大肠杆菌中进行克级规模的甲硫氨酰 - tRNA合成酶和酪氨酰 - tRNA合成酶的纯化。
Gram-scale purification of methionyl-tRNA and tyrosyl-tRNA synthetases from Escherichia coli.
作者信息
Bruton C, Jakes R, Atkinson T
出版信息
Eur J Biochem. 1975 Nov 15;59(2):327-33. doi: 10.1111/j.1432-1033.1975.tb02459.x.
Abstract
A procedure is presented for the purification of 3 g of homogeneous methionyl-tRNA synthetase and 1 g of homogeneous tyrosyl-tRNA synthetase from 50 kg batches of Escherichia coli. The procedure permits the isolation of many enzymes simultaneously and the elution positions of seven other aminoacyl-tRNA synthetases, catalase, rhodanese, phosphofructokinase, elongation factor Tu and cytochrome b-562 are indicated. The problems of extraction work on this scale are discussed.
摘要
本文介绍了一种从50千克大肠杆菌中纯化3克均一的甲硫氨酰 - tRNA合成酶和1克均一的酪氨酰 - tRNA合成酶的方法。该方法可同时分离多种酶，并指出了其他七种氨酰 - tRNA合成酶、过氧化氢酶、硫氰酸酶、磷酸果糖激酶、延伸因子Tu和细胞色素b - 562的洗脱位置。讨论了这种规模提取工作的相关问题。
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