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Purification and properties of tyrosyl tRNA synthetase of rat liver.

作者信息

Prasada Rao Y S, Srinivasan P R

出版信息

Nucleic Acids Res. 1977 Nov;4(11):3887-900. doi: 10.1093/nar/4.11.3887.

DOI:10.1093/nar/4.11.3887
PMID:593893
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343208/
Abstract

Purification of rat liver tyrosine tRNA synthetase yields two protein fractions A and B and both fractions are required for charging of tyrosine to tRNAtyr. Fraction B catalyzes the activation of tyrosine. Fractions A and B have been purified to near homogeneity and they are composed of single polypeptide chains of 62,000 daltons each. Gel filtration studies suggest a molecular weight of 120,000 for the synthetase.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dadc/343208/d7eb2cdd6d6f/nar00484-0221-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dadc/343208/d7eb2cdd6d6f/nar00484-0221-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dadc/343208/d7eb2cdd6d6f/nar00484-0221-a.jpg

相似文献

1
Purification and properties of tyrosyl tRNA synthetase of rat liver.
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本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
The acetate activating enzyme system of heart muscle.心肌中的乙酸激活酶系统。
J Biol Chem. 1953 Jul;203(1):35-45.
3
Comparison of transfer RNA and aminoacyl RNA ligases from calf liver and spleen.来自小牛肝脏和脾脏的转运RNA及氨酰RNA连接酶的比较。
Biochim Biophys Acta. 1966 Sep;123(3):503-9. doi: 10.1016/0005-2787(66)90218-8.
4
[Ion-exchange sorption of radioactive acylhydroxamates as a method for determination of aminoacyl-tRNA synthetase activity].
Biokhimiia. 1967 Jul-Aug;32(4):735-40.
5
Purification and physical characterization of tyrosyl ribonucleic acid synthetases from Escherichia coli and Bacillus subtilis.来自大肠杆菌和枯草芽孢杆菌的酪氨酰核糖核酸合成酶的纯化及物理特性研究
Biochemistry. 1966 May;5(5):1681-90. doi: 10.1021/bi00869a033.
6
Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.通过SDS-聚丙烯酰胺凝胶电泳对多肽链进行分子量估计
Biochem Biophys Res Commun. 1967 Sep 7;28(5):815-20. doi: 10.1016/0006-291x(67)90391-9.
7
Tyrosyl transfer ribonucleic acid synthetase from Bacillus stearothermophilus. Preparation and properties of the crystallizable enzyme.
Biochemistry. 1974 May 21;13(11):2307-12. doi: 10.1021/bi00708a011.
8
Purification of tyrosine: tRNA ligase from Saccharomyces cerevisiae alphaS288C.
J Biochem. 1973 Apr;73(4):811-9. doi: 10.1093/oxfordjournals.jbchem.a130144.
9
Tyrosyl-tRNA synthetase of Escherichia coli B. Binding of various ligands.大肠杆菌B的酪氨酰-tRNA合成酶。各种配体的结合
Eur J Biochem. 1973 May;35(1):51-6. doi: 10.1111/j.1432-1033.1973.tb02808.x.
10
Glutamyl transfer ribonucleic acid synthetase of Escherichia coli. 3. Influence of the 46K protein on the affinity of the 56K glutamyl transfer ribonucleic acid synthetase for its substrates.
J Biol Chem. 1972 Aug 25;247(16):4982-5.