Rastogi Amit, Srivastava Pradeep, Iqbal Zafer, Kumaraswamy Vinay, Singh Ravindra Pratap
Department of Orthopaedics, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
Indian J Orthop. 2013 Mar;47(2):129-34. doi: 10.4103/0019-5413.108878.
Injuries of articular cartilage (AC) have very limited potential to heal, because they are avascular and this may subsequently lead to secondary arthrosis. Autologous cultured chondrocytes transplantation is can be used to create hyaline or hyaline-like repair in a cartilage defect area. The purpose of this study was to repair artificially created full-thickness AC defects in 20 rabbit knee joints with autologous cultured chondrocytes.
An AC defect of 3 mm was created on the lateral condyle of both tibiae. The defect was filled with autologous chondrocytes cultured in vitro and fixed with fibrin, at a later stage on the left side. The right knee acted as a control. The rabbits were sacrificed after 3, 6, and 12 weeks of transplantation and the reparative tissues were analyzed macroscopically and histologically.
Histological scores of the cultured autologous chondrocyte transplanted knees were significantly better than the control knees at 3, 6, and 12 weeks following the transplantation. Integration of repaired tissue with adjacent cartilage, hyaline characteristics of repaired tissue, maturity of cartilage, and cellularity increases with duration and is significant in chondrocytes-transplanted defects compared to control. The histological scores also become better with increasing duration of followup.
Transplantation of autologous chondrocytes cultured in vitro and fixed with fibrin is effective in repairing AC defects.
关节软骨损伤的愈合潜力非常有限，因为它们无血管，这随后可能导致继发性关节炎。自体培养软骨细胞移植可用于在软骨缺损区域形成透明软骨或类似透明软骨的修复组织。本研究的目的是用自体培养软骨细胞修复20只兔膝关节人工制造的全层关节软骨缺损。
在双侧胫骨外侧髁制造3毫米的关节软骨缺损。缺损处用体外培养的自体软骨细胞填充并用纤维蛋白固定，后期在左侧进行。右膝作为对照。移植后3周、6周和12周处死兔子，对修复组织进行宏观和组织学分析。
在移植后3周、6周和12周，培养的自体软骨细胞移植膝关节的组织学评分明显优于对照膝关节。修复组织与相邻软骨的整合、修复组织的透明软骨特征、软骨成熟度和细胞密度随时间增加，与对照相比，在软骨细胞移植缺损中显著。组织学评分也随着随访时间的延长而改善。
体外培养并用纤维蛋白固定的自体软骨细胞移植对修复关节软骨缺损有效。
