德黑兰阴道毛滴虫分离株中的双链RNA病毒感染
Double-Stranded RNA Viral Infection in Tehran Trichomonas vaginalis Isolates.
作者信息
Heidary S, Bandehpour M, Valadkhani Z, Seyyed-Tabaee Sj, Haghighi A, Abadi Ar, Kazemi B
机构信息
Dept. of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
Iran J Parasitol. 2013 Jan;8(1):60-4.
BACKGROUND
Trichomonas vaginalis is a pathogenic protozoon and may be contaminated with dsRNA virus called Trichomonas vaginalis virus (TVV). The viral infection is an important factor for its pathogenesis and sensitivity to metronidazole. The presence of TVV is associated with qualitative and quantitative expression of cysteine proteinases and surface immunogenic; P270. The purpose of this study was to determine TVV frequency in T. vaginalis clinical isolates in Tehran, Iran.
METHODS
The 46 T. vaginalis isolates were collected from Tehran Province and cultured in TYI-S-33 culture medium. Viral RNA was extracted and RT-PCR was done.
RESULTS
Of 46 T. vaginalis isolates, 8 isolates (17.39%) were infected with TVV-1. There was not any association between patient age and TVV- infected T. vaginalis. There were 17.39% viral infection in T. vaginalis isolates which was lower than that reported by other researchers.
CONCLUSION
This is the first report on T. vaginalis isolates infection by TVV-1 in Iran.
背景
阴道毛滴虫是一种致病原生动物，可能感染一种名为阴道毛滴虫病毒（TVV）的双链RNA病毒。病毒感染是其发病机制和对甲硝唑敏感性的重要因素。TVV的存在与半胱氨酸蛋白酶的定性和定量表达以及表面免疫原性P270有关。本研究的目的是确定伊朗德黑兰阴道毛滴虫临床分离株中TVV的感染频率。
方法
从德黑兰省收集46株阴道毛滴虫分离株，并在TYI-S-33培养基中培养。提取病毒RNA并进行逆转录聚合酶链反应（RT-PCR）。
结果
在46株阴道毛滴虫分离株中，8株（17.39%）感染了TVV-1。患者年龄与感染TVV的阴道毛滴虫之间没有关联。阴道毛滴虫分离株中有17.39%发生病毒感染，低于其他研究人员报告的感染率。
结论
这是伊朗关于TVV-1感染阴道毛滴虫分离株的首次报告。
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