在伊朗分离株中首次检测到双链RNA病毒1、2和3的共感染。
The First Detection of Co-Infection of Double-Stranded RNA Virus 1, 2 and 3 in Iranian Isolates of .
作者信息
Bokharaei-Salim Farah, Esteghamati Abdoulreza, Khanaliha Khadijeh, Esghaei Maryam, Donyavi Tahereh, Salemi Borna
机构信息
Department of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran.
出版信息
Iran J Parasitol. 2020 Jul-Sep;15(3):357-363. doi: 10.18502/ijpa.v15i3.4200.
BACKGROUND
The Totiviridae family includes a number of double-stranded RNA viruses that can infect . Some isolates are infected with one or more double-stranded RNA (dsRNA) viruses. In this study, different strains of double-stranded RNA virus in Iranian isolates of were evaluated for the first time in Iran.
METHODS
Vaginal swabs were collected from 1550 participants who were referred to hospitals associated with Iran University of Medical Sciences, Tehran, Iran from June to November 2018. isolates were cultured in Diamond's modified medium. After the extraction of nucleic acids using a DNA/RNA extraction kit, RT-PCR was performed and PCR products were purified and sequenced.
RESULTS
In general 9 (0.6%) isolates were confirmed as among 1550 collected vaginal samples. Among 9 isolates of , three of them were infected with TVV1. One isolate has multiple infections with virus (TVV1, TVV2 and TVV3) as coinfection. The nucleotide BLAST indicated that the virus 1(TVV1) isolates were most closely related to TVV1-OC5, TVV1-UR1-1.The virus 2 (TVV2) sequence had also a similarity with TVV2-UR1-1, TVV2-UR1 and TVV2-OC3. The sequence of virus 3(TVV3) had similarity with TVV3-OC5, TVV3-UR1-1 and TVV3-UR1.
CONCLUSION
Three dsRNA viruses virus (TVV1, TVV2 and TVV3) were detected using RT-PCR in Iranian isolates. The coinfection of TVV1, TVV2 and TVV3 in one isolate of was observed for the first time in Iran.
背景
双链核糖核酸病毒科包含多种可感染……的双链核糖核酸病毒。一些……分离株感染了一种或多种双链核糖核酸（dsRNA）病毒。在本研究中，伊朗首次对……伊朗分离株中的不同双链核糖核酸病毒株进行了评估。
方法
2018年6月至11月，从1550名转诊至伊朗德黑兰医科大学附属医院的参与者中采集阴道拭子。……分离株在改良的戴蒙德培养基中培养。使用DNA/RNA提取试剂盒提取核酸后，进行逆转录聚合酶链反应（RT-PCR），并对PCR产物进行纯化和测序。
结果
在1550份采集的阴道样本中，总体上有9份（0.6%）分离株被确认为……。在9份……分离株中，3份感染了TVV1。1份分离株存在病毒多重感染（TVV1、TVV2和TVV3），为混合感染。核苷酸BLAST分析表明，双链核糖核酸病毒1（TVV1）分离株与TVV1-OC5、TVV1-UR1-1关系最为密切。双链核糖核酸病毒2（TVV2）序列也与TVV2-UR1-1、TVV2-UR1和TVV2-OC3相似。双链核糖核酸病毒3（TVV3）序列与TVV3-OC5、TVV3-UR1-1和TVV3-UR1相似。
结论
使用RT-PCR在……伊朗分离株中检测到3种双链核糖核酸病毒（TVV1、TVV2和TVV3）。伊朗首次观察到1份……分离株中存在TVV1、TVV2和TVV3的混合感染。
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