利用直接凝集试验对伊朗中部阿尔伯兹省流浪犬和家养犬内脏利什曼病进行血清流行病学调查。
Seroepidemiologcal investigation of visceral leishmaniasis in stray and owned dogs in alborz province, central iran using direct agglutination test.
作者信息
Haddadzade Hr, Fattahi R, Mohebali M, Akhoundi B, Ebrahimzade E
机构信息
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran,Iran.
出版信息
Iran J Parasitol. 2013 Jan;8(1):152-7.
BACKGROUND
The aim of present study was to determine the seroprevalence of canine visceral leishmaniasis (CVL) among stray and owned dogs in Kouhsar district of Alborz Province, central Iran.
METHODS
The study was performed from March 2011 to July 2011 using Direct Agglutination Test (DAT). Three hundred and thirty seven dogs including 257 stary and 80 owned dogs were selected by random sampling. The agreement between serological data and sex, age, life style of dogs and clinical signs were assessed by Chi-square.
RESULTS
DAT showed that from 337 serum samples collected from owned and stray dogs, 12sera (3.6%) were positive. The seroprevalance was 10% (8/80) among owned dogs and 1.6% (4/257) among stray dogs. A significant difference in seroplevalance was seen between owned and stray dogs (P = 0.01). The highest seroprevalence rate (14%) was observed among the ownership dogs of 5 years old and above. Statistical analysis revealed significant relation between seroprelvalence and age (P= 0.02). There was no statistically significant relation between male (6.3%) and female (2.2%) seroprevalence (P= 0.085).
CONCLUSION
This survey indicates the importance and necessity of serologic screening of visceral leishmaniasis in human and dogs in Kouhsar district.
背景
本研究旨在确定伊朗中部阿尔伯兹省库萨尔地区流浪犬和家养犬中犬内脏利什曼病（CVL）的血清阳性率。
方法
本研究于2011年3月至2011年7月使用直接凝集试验（DAT）进行。通过随机抽样选择了337只狗，其中包括257只流浪犬和80只家养犬。通过卡方检验评估血清学数据与狗的性别、年龄、生活方式和临床症状之间的一致性。
结果
DAT显示，从家养犬和流浪犬采集的337份血清样本中，有12份血清（3.6%）呈阳性。家养犬中的血清阳性率为10%（8/80），流浪犬中的血清阳性率为1.6%（4/257）。家养犬和流浪犬之间的血清阳性率存在显著差异（P = 0.01）。在5岁及以上的家养犬中观察到最高血清阳性率（14%）。统计分析显示血清阳性率与年龄之间存在显著关系（P = 0.02）。雄性（6.3%）和雌性（2.2%）的血清阳性率之间没有统计学上的显著关系（P = 0.085）。
结论
本次调查表明在库萨尔地区对人和犬进行内脏利什曼病血清学筛查的重要性和必要性。
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