Heidari Aliehsan, Mohebali Mehdi, Vahed Mozhgan, Kabir Kourosh, Zarei Zabihollah, Akhoundi Behnaz, Elikaee Samira, Barati Hojatallah, Sezavar Monireh, Keshavarz Hossein, Kakooei Zahra, Hajjaran Homa
Department of Parasitology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
J Arthropod Borne Dis. 2020 Mar 31;14(1):38-46. doi: 10.18502/jad.v14i1.2702. eCollection 2020 Mar.
Mediterranean form of visceral leishmaniasis (VL) is endemic among some provinces of Iran. The present study was designed to determine the prevalence of canine visceral leishmaniasis (CVL) in the owned dogs of the rural areas of Alborz Province near Tehran as the capital of Iran.
This study conducted on 303 owned dogs that selected using a stratified random sampling method. The direct agglutination test (DAT) was used to determine the frequency of Vl. The spleen biopsy was taken from the serology-positive dogs for the confirmation of CVL in the suspected dogs. Nested PCR and sequencing methods were used to determine the type of species in the dogs which were parasitological positive.
Overall, the DAT results of 9 dogs (2.97%, CI: 1.57-5.55) showed anti antibodies at titers ≥ 1:320 indicating VL infection. One dog (0.33%, CI 95%: 0.06-1.85) showed clinical signs and symptoms of VL. There was a significant correlation between the positive cases of CVL and rural area (p< 0.001). The was observed in the impression smears that were prepared from spleen biopsy of five the studied dogs. were confirmed in all them using nested-PCR assay. The sequence analysis of all five isolates was 95% similar to .
This study shows that domestic cycle of has been established in rural areas of Alborz province where located near Tehran as capital city of Iran. It is necessary to increase the awareness and monitoring of the disease periodically.
地中海型内脏利什曼病（VL）在伊朗的一些省份呈地方流行。本研究旨在确定伊朗首都德黑兰附近阿尔伯兹省农村地区家养犬中犬内脏利什曼病（CVL）的患病率。
本研究对303只采用分层随机抽样方法选取的家养犬进行。直接凝集试验（DAT）用于确定VL的感染频率。对血清学阳性犬进行脾脏活检，以确诊疑似犬的CVL。采用巢式PCR和测序方法确定寄生虫学阳性犬的物种类型。
总体而言，9只犬（2.97%，CI：1.57 - 5.55）的DAT结果显示抗体滴度≥1:320，表明感染了VL。1只犬（0.33%，95%CI：0.06 - 1.85）出现了VL的临床症状和体征。CVL阳性病例与农村地区之间存在显著相关性（p < 0.001）。在对5只研究犬的脾脏活检制备的印片中观察到了[此处原文缺失相关内容]。通过巢式PCR检测在所有犬中均得到证实。对所有5株分离株的序列分析显示与[此处原文缺失相关内容]相似度为95%。
本研究表明，在伊朗首都德黑兰附近的阿尔伯兹省农村地区已建立了[此处原文缺失相关内容]的家庭传播循环。有必要提高对该疾病的认识并定期进行监测。
