Weliky N, Leaman D H, Kallman B J
Cancer Res. 1975 Jun;35(6):1580-5.
The soluble complement-fixing antigen of the P3HR-1 Burkitt's lymphoma cell line, identified with human serum containing antibody against Epstein-Barr virus viral capsid antigen, loses activity under a variety of conditions. The major characteristic reported here is retention of activity on exposure to specific physical or chemical conditions followed by loss of activity after subsequent neutral dialysis. Antigen of untreated cell lysates, which retains activity after dialysis against large volumes of neutral buffers or 0.14 M NaCl, will lose activity if the lysate is first heated to 56 degrees or if the lysate is exposed to acid perchlorate, and the resulting precipitate and supernatant are redialyzed separately to neutral pH. The P3HR-1 soluble complement-fixing antigen appears to be an aggregate including a labile, dissociable component.
用含抗爱泼斯坦 - 巴尔病毒衣壳抗原抗体的人血清鉴定出的P3HR - 1伯基特淋巴瘤细胞系的可溶性补体结合抗原，在多种条件下会失去活性。此处报道的主要特征是，在暴露于特定物理或化学条件下时抗原保留活性，而在随后的中性透析后失去活性。未经处理的细胞裂解物的抗原，在针对大量中性缓冲液或0.14M NaCl透析后仍保留活性，但如果裂解物先加热至56℃，或者裂解物暴露于高氯酸，然后将所得沉淀和上清液分别重新透析至中性pH，则会失去活性。P3HR - 1可溶性补体结合抗原似乎是一种聚集体，包括一个不稳定、可解离的成分。
