Weliky N, Leaman D H, Kallman B J
J Natl Cancer Inst. 1975 Mar;54(3):593-6.
Soluble complement-fixing (CF) antigens of the virus-producing and virus-nonproducing P3HR-1 and RAJI Burkitt lymphoma cell lines and Hk-Ly-28 nasopharyngeal cancer cell line, identified by use of viral capsid antigen-positive human sera, could readily be distinguished by differences in their stability to chemical and physical conditions. The P3HR-1 soluble CF antigen lost titer when heated or exposed to acid perchlorate under conditions in which RAJI and Hk-Ly-28 soluble antigens were stable. Differences in solubility were also found. These results contribute not only to the interpretation of the relationship of Epstein-Barr virus (EBV)-associated components of RAJI to P3HR-1 and Hk-Ly-28 cell lysates but also to the selection of conditions for the development of identification tests and purification procedures for CF antigens and antibodies of Burkitt's lymphoma, nasopharyngeal cancer, and other EBV-associated tumors.
通过使用病毒衣壳抗原阳性的人血清鉴定出，产生病毒和不产生病毒的P3HR - 1和RAJI伯基特淋巴瘤细胞系以及Hk - Ly - 28鼻咽癌细胞系的可溶性补体结合（CF）抗原，可根据它们对化学和物理条件稳定性的差异轻易区分。在RAJI和Hk - Ly - 28可溶性抗原稳定的条件下，P3HR - 1可溶性CF抗原在加热或暴露于高氯酸时滴度会降低。还发现了溶解度的差异。这些结果不仅有助于解释RAJI中与爱泼斯坦 - 巴尔病毒（EBV）相关成分与P3HR - 1和Hk - Ly - 28细胞裂解物之间的关系，也有助于为伯基特淋巴瘤、鼻咽癌和其他EBV相关肿瘤的CF抗原及抗体的鉴定试验和纯化程序的开发选择条件。
