Thurow H, Choy Y M, Frank N, Niemann H, Stirm S
Carbohydr Res. 1975 May;41:241-55. doi: 10.1016/s0008-6215(00)87023-8.
Using periodate oxidation, methylation analysis, the characterization of oligosaccharides obtained by partial acid hydrolysis, p.m.r. spectroscopy, and analytical ultracentrifugation, the structure of the (mildly alkali-treated) Klebsiella serotype 11 capusular polysaccharide has been elucidated. The tetrasaccharide repeating-unit comprises the sequence yields 3)-beta-D-Glcp-(1 yields 3)-beta-D-GlcUAp-(1 yields 3)-alpha-D-Galp-(1 yields with a 4,6-O-(1-carboxyethylidene)-alpha-D-galactosyl residue linked to O-4 of the glucuronic acid residue. The structural basis for some serological cross-reactions of the Klebseilla K11 antigen is discussed, and it is shown that rabbit antisera against the Klebsiella K11 test-strain predominantly contain K agglutinins specific for branch-terminal 4,6-O-(1-carboxyethylidene)-D-galactose.
通过高碘酸盐氧化、甲基化分析、部分酸水解所得寡糖的表征、核磁共振光谱以及分析超速离心法，已阐明了（轻度碱处理的）肺炎克雷伯菌11型荚膜多糖的结构。四糖重复单元包含以下序列：（1→3）-β-D-葡萄糖吡喃糖基-（1→3）-β-D-葡萄糖醛酸吡喃糖基-（1→3）-α-D-半乳糖吡喃糖基-（1→ ），其中一个4,6-O-（1-羧基亚乙基）-α-D-半乳糖基残基连接到葡萄糖醛酸残基的O-4上。讨论了肺炎克雷伯菌K11抗原一些血清学交叉反应的结构基础，并表明针对肺炎克雷伯菌K11测试菌株的兔抗血清主要含有对分支末端4,6-O-（1-羧基亚乙基）-D-半乳糖特异的K凝集素。
