Department of Clinical Pathomorphology, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, 9 Curie Skłodowska Street, 85-094 Bydgoszcz, Poland.
Acta Histochem. 2013 Oct;115(8):775-82. doi: 10.1016/j.acthis.2013.04.002. Epub 2013 May 15.
Actin is one of the most abundant cytoskeletal proteins, which takes part in many cellular processes. This review provides information on the history, forms and localization of actin and its role, in particular in cellular death processes. We discuss the relationships between reorganization of actin filaments and apoptosis, mitotic catastrophe and differentiation. Finally, we discuss the translocation and accumulation of actin in the nuclear area. Moreover, owing to the difficulties of F-actin localization by transmission electron microscopy (TEM), the phalloidin-based method of its detection using streptavidin-coated quantum dots is presented in this review.
肌动蛋白是细胞骨架中含量最丰富的蛋白之一，参与了许多细胞过程。本文综述了肌动蛋白的历史、形式和定位及其作用，特别是在细胞死亡过程中的作用。我们讨论了肌动蛋白丝重组与细胞凋亡、有丝分裂灾难和分化之间的关系。最后，我们讨论了肌动蛋白在核区的易位和积累。此外，由于透射电镜（TEM）对 F-肌动蛋白定位的困难，本文还介绍了使用链霉亲和素包被的量子点检测 F-肌动蛋白的鬼笔环肽法。
