Alexiou George A, Vartholomatos Evrysthenis, Goussia Anna, Dova Leukothea, Karamoutsios Achilleas, Fotakopoulos George, Kyritsis Athanasios P, Voulgaris Spyridon
Department of Neurosurgery, University Hospital of Ioannina, Ioannina, Greece.
Clin Neurol Neurosurg. 2013 Sep;115(9):1784-7. doi: 10.1016/j.clineuro.2013.04.015. Epub 2013 May 15.
Flow cytometry has been applied to analyze the DNA-content distribution of tumors, in order to relate this to clinical and biological parameters of tumor behavior. Herewith, we investigated the value of cell cycle analysis in the characterization of intracranial lesions and its possible prognostic role.
DNA analysis was performed in tumor samples that were taken during surgery over a five year period. Diagnosed tumors were graded according to the World Health Organization 2007 classification scheme.
Fifty-six patients were included in the study. There was a significant difference in G0/G1 phase and S-phase between low-grade and high-grade gliomas. There were 12 (57%) diploid and 9 (43%) aneuploid tumors. All aneuploid tumors were glioblastomas. Patients with G0/G1 value ≤ 69% and S phase value greater than 6% were associated with worse survival. As regards meningiomas, there was a significant difference in G0/G1 phase, S phase and mitoses fraction between benign and both atypical and anaplastic meningiomas. Aneuploidy was observed in the anaplastic tumors and in 2/4 atypical meningiomas.
The results of the present study, showed that cell cycle analysis could differentiate low from high grade gliomas and benign from atypical/anaplastic meningiomas. Furthermore, a prognostic significance was found in glioma patients. The role of cell cycle analysis in brain tumors thus warrants further investigation.
流式细胞术已被用于分析肿瘤的DNA含量分布，以便将其与肿瘤行为的临床和生物学参数相关联。据此，我们研究了细胞周期分析在颅内病变特征描述中的价值及其可能的预后作用。
对五年期间手术获取的肿瘤样本进行DNA分析。根据世界卫生组织2007年分类方案对诊断出的肿瘤进行分级。
56例患者纳入研究。低级别和高级别胶质瘤在G0/G1期和S期存在显著差异。有12例（57%）二倍体肿瘤和9例（43%）非整倍体肿瘤。所有非整倍体肿瘤均为胶质母细胞瘤。G0/G1值≤69%且S期值大于6%的患者生存较差。对于脑膜瘤，良性与非典型和间变性脑膜瘤在G0/G1期、S期和有丝分裂分数方面存在显著差异。在间变性肿瘤和2/4例非典型脑膜瘤中观察到非整倍体。
本研究结果表明，细胞周期分析可区分低级别和高级别胶质瘤以及良性与非典型/间变性脑膜瘤。此外，在胶质瘤患者中发现了预后意义。因此，细胞周期分析在脑肿瘤中的作用值得进一步研究。
