皮质醇与培养的鸡胚神经视网膜的相互作用。

Interaction of cortisol with the neural retina of the chick embryo in culture.

作者信息

Diamant A, Eshel M, Ben-Or S

出版信息

Cell Differ. 1975 May;4(2):101-12. doi: 10.1016/0045-6039(75)90022-6.

Abstract

The uptake of cortisol and the kinetics of hormone-receptor interaction in the cytosol and cell nucleus were investigated in the intact tissue in organ culture. Cortisol is concentrated by the neural retina. The accumulation of the free steroid is temperature dependent but the effect of temperature decreases with the increase of cortisol in medium. Cortisol binding to specific receptors in the cytosol shows a sigmoidal type of kinetics which correlates well with the kinetics of glutamine-synthetase induction by cortisol. The temperature dependent translocation of the receptor-hormone complexes to the nuclei and the effect of detergents on the binding to nuclei are presented.

摘要

在器官培养的完整组织中，研究了皮质醇的摄取以及激素受体在细胞质和细胞核中的相互作用动力学。神经视网膜可浓缩皮质醇。游离类固醇的积累取决于温度，但随着培养基中皮质醇浓度的增加，温度的影响会降低。皮质醇与细胞质中特定受体的结合呈现出S型动力学，这与皮质醇诱导谷氨酰胺合成酶的动力学密切相关。本文还介绍了受体-激素复合物向细胞核的温度依赖性转运以及去污剂对其与细胞核结合的影响。