Griesmacher A, Müller M M
Abteilung Klinische Biochemie, Chirurgischen Universitätsklinik Wien, Osterreich.
Z Med Lab Diagn. 1990;31(3):139-46.
In human umbilical endothelial cells purine metabolism was studied: activities of some key-enzymes, concentrations of ATP, ADP, and creatine phosphate (CP), and the incorporation of labeled purine bases, purine nucleosides and glycine were measured. The results obtained show that endothelial cells are capable of degradation and synthesis of purine nucleotides. The rate of purine nucleotide synthesis via salvage pathways predominates that of de novo synthesis. Preincubation with phosphate resulted in increased nucleotide pools and incorporation of performed purines, whereas ribose showed the opposite effect. An explanation of these effects might be the limited availability of intracellular phosphoribosylpyrophosphate.
测定了一些关键酶的活性、三磷酸腺苷（ATP）、二磷酸腺苷（ADP）和磷酸肌酸（CP）的浓度，以及标记嘌呤碱基、嘌呤核苷和甘氨酸的掺入情况。所得结果表明，内皮细胞能够降解和合成嘌呤核苷酸。通过补救途径合成嘌呤核苷酸的速率高于从头合成的速率。用磷酸盐预孵育导致核苷酸池增加和已形成嘌呤的掺入，而核糖则表现出相反的效果。这些效应的一种解释可能是细胞内磷酸核糖焦磷酸的可用性有限。
