Department of Veterinary Microbiology, School of Veterinary Medicine, Faculty of Agriculture, Iwate University, 18-8 Ueda 3, Chome, Morioka, 020-8550 Iwate, Japan.
J Virol Methods. 2013 Sep;192(1-2):59-67. doi: 10.1016/j.jviromet.2013.04.023. Epub 2013 May 14.
The genus Pestivirus from the family Flaviviridae is represented by four established species; Bovine viral diarrhea virus 1 (BVDV-1); Bovine viral diarrhea virus 2 (BVDV-2); Border disease virus (BDV); and Classical swine fever virus (CSFV); as well a tentative species from a Giraffe. The palindromic nucleotide substitutions (PNS) in the 5' untranslated region (UTR) of Pestivirus RNA has been described as a new, simple and practical method for genotyping. New software is described, also named PNS, that was prepared specifically for this PNS genotyping procedure. Pestivirus identification using PNS was evaluated on five hundred and forty-three sequences at genus, species and genotype level using this software. The software is freely available at www.pns-software.com.
黄病毒科瘟病毒属包括四个已确定的种，即牛病毒性腹泻病毒 1 型（BVDV-1）、牛病毒性腹泻病毒 2 型（BVDV-2）、边界病病毒（BDV）和古典猪瘟病毒（CSFV），以及一种来自长颈鹿的暂定种。瘟病毒 RNA 的 5'非翻译区（UTR）中的回文核苷酸替换（PNS）已被描述为一种新的、简单实用的基因分型方法。本文还描述了一种新的软件，也称为 PNS，它是专门为这种 PNS 基因分型程序准备的。使用该软件，在属、种和基因型水平上对 543 个序列进行了瘟病毒 PNS 鉴定。该软件可在 www.pns-software.com 上免费获得。
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