van Rijn P A, van Gennip H G, Leendertse C H, Bruschke C J, Paton D J, Moormann R J, van Oirschot J T
Department of Mammalian Virology, Institute for Animal Science and Health, Lelystad, 8200 AB, The Netherlands.
Virology. 1997 Oct 27;237(2):337-48. doi: 10.1006/viro.1997.8792.
Conventionally, the genus Pestivirus of the family Flaviviridae has been divided into bovine viral diarrhea virus (BVDV), classical swine fever virus (CSFV), and border disease virus (BDV). To date, BDV and BVDV have been isolated from different species, whereas CSFV seems to be restricted to swine. Pestiviruses are structurally and antigenically closely related. Envelope glycoprotein E2 is the most immunogenic and most variable protein of pestiviruses. We cloned E2 genes of many different pestivirus strains, including those from a deer and a giraffe. The E2 genes were transiently expressed, characterized with monoclonal antibodies, sequenced, and compared. Based on these data, we can delineate six major groups within the Pestivirus genus. Four groups correspond to defined genotypes, whereas the two other groups could be new genotypes within the Pestivirus genus. One group comprises CSFV strains isolated from swine. A second group consists of BDV strains Moredun, L83, and X818, which have been isolated from sheep, and strain F from swine. A third group contains strain BD78 from sheep, strain 5250 from swine, and strain 178003 from cattle. On the basis of E2, these viruses are very similar to BVDV strains associated with acute severe outbreaks of bovine viral diarrhea, so-called type 2 BVDV. The fourth group consists of BVDV strains originating predominantly from cattle. This BVDV group can be divided into two subtypes or subgroups BVDV Ia and Ib: BVDV Ia contains viruses from the United States, such as like NADL and Oregon, and some others, such as 150022 and 1138 from Europe. Subgroup BVDV Ib contains strain Osloss and several Dutch isolates. The fifth and sixth "groups" could be proposed as two new genotypes and contain strains Deer and Giraffe, respectively.
传统上，黄病毒科瘟病毒属分为牛病毒性腹泻病毒（BVDV）、经典猪瘟病毒（CSFV）和边境病病毒（BDV）。迄今为止，BDV和BVDV已从不同物种中分离出来，而CSFV似乎仅局限于猪。瘟病毒在结构和抗原性上密切相关。包膜糖蛋白E2是瘟病毒中免疫原性最强且变异最大的蛋白。我们克隆了许多不同瘟病毒株的E2基因，包括来自鹿和长颈鹿的毒株。这些E2基因被瞬时表达，用单克隆抗体进行表征，测序并比较。基于这些数据，我们可以在瘟病毒属内划分出六个主要组。其中四个组对应于已确定的基因型，而另外两个组可能是瘟病毒属内的新基因型。一组包括从猪分离出的CSFV毒株。第二组由从绵羊分离出的BDV毒株莫雷顿、L83和X818以及从猪分离出的F毒株组成。第三组包含来自绵羊的BD78毒株、来自猪的5250毒株和来自牛的178003毒株。基于E2，这些病毒与与牛病毒性腹泻急性严重暴发相关的BVDV毒株非常相似，即所谓的2型BVDV。第四组由主要源自牛的BVDV毒株组成。这个BVDV组可分为两个亚型或亚组BVDV Ia和Ib：BVDV Ia包含来自美国的病毒，如NADL和俄勒冈毒株，以及其他一些病毒，如来自欧洲的150022和1138毒株。亚组BVDV Ib包含奥洛斯毒株和几个荷兰分离株。第五和第六个“组”可被提议为两个新基因型，分别包含鹿和长颈鹿毒株。
