热量限制对大鼠急性乙醇诱导的氧化和硝化肝损伤的影响。
The effect of calorie restriction on acute ethanol-induced oxidative and nitrosative liver injury in rats.
机构信息
Institute of Pathophysiology, "Ljubodrag Buba Mihailović", Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
Institute for Medical Research, Military Medical Academy, Belgrade, Serbia.
出版信息
Environ Toxicol Pharmacol. 2013 Sep;36(2):296-302. doi: 10.1016/j.etap.2013.04.002. Epub 2013 Apr 17.
The aim of our study was to examine the effect of calorie restriction (CR) on oxidative and nitrosative liver injury in rats, induced by acute ethanol intoxication. Male Wistar rats were divided into groups: (1) control; (2) calorie-restricted groups with intake of 60-70% (CR60-70) and 40-50% of daily energy needs (CR40-50); (3) ethanol-treated group (E); (4) calorie-restricted, ethanol-treated groups (E+CR60-70 and E+CR40-50). Ethanol was administered in 5 doses of 2g/kg every 12h, and duration of CR was 5 weeks before ethanol treatment. Malondialdehyde and nitrite and nitrate level were significantly lower in E+CR60-70 and higher in E+CR40-50 vs. E group. Liver reduced glutathione content and activity of both superoxide dismutase izoenzymes were significantly higher in E+CR60-70 and lower in E+CR40-50 vs. E group. Oxidative stress may be a potential mechanism of hormetic effects of CR on acute ethanol-induced liver injury.
我们的研究目的是检验热量限制（CR）对急性乙醇中毒诱导的大鼠氧化和硝化肝损伤的影响。雄性 Wistar 大鼠分为以下几组：（1）对照组；（2）热量限制组，能量摄入为每日需求的 60-70%（CR60-70）和 40-50%（CR40-50）；（3）乙醇处理组（E）；（4）热量限制、乙醇处理组（E+CR60-70 和 E+CR40-50）。乙醇以 2g/kg 的 5 个剂量每 12 小时给予，CR 持续 5 周，然后进行乙醇处理。与 E 组相比，E+CR60-70 组的丙二醛和亚硝酸盐/硝酸盐水平显著降低，E+CR40-50 组显著升高。E+CR60-70 组的肝还原型谷胱甘肽含量和两种超氧化物歧化酶同工酶的活性均显著升高，而 E+CR40-50 组则显著降低。氧化应激可能是 CR 对急性乙醇诱导的肝损伤产生适应效应的潜在机制。
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