State Key Laboratory of Virology and the Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, PR China.
J Cell Biochem. 2013 Oct;114(10):2323-33. doi: 10.1002/jcb.24579.
Zinc-fingers and homeoboxes protein 1 (ZHX1) belongs to the ZF (zinc-fingers) class of homeodomain transcription factors, and its function remains largely unknown. ZHX1 has been previously found to interact with the activation domain of the nuclear factor Y subunit A (NFYA) and to have a transcriptional repression activity. Here, we report that the SUMO-E2 conjugating enzyme Ubc9 was identified to interact with ZHX1 by an interaction screen using a yeast two-hybrid system. This interaction was confirmed by co-immunoprecipitation and co-localization assays. Further study showed that ZHX1 is SUMOylated by Ubc9 with SUMO1 at the sites K159, K454, and K626. Furthermore, we demonstrated that the SUMOylation of ZHX1 regulated the stability, ubiquitination and transcriptional activity of ZHX1.
锌指和同源盒蛋白 1（ZHX1）属于锌指（ZF）类同源盒转录因子，其功能在很大程度上尚不清楚。先前已经发现 ZHX1 与核因子 Y 亚基 A（NFYA）的激活结构域相互作用，并具有转录抑制活性。在这里，我们报告说，SUMO-E2 连接酶 Ubc9 通过使用酵母双杂交系统的相互作用筛选被鉴定与 ZHX1 相互作用。通过共免疫沉淀和共定位测定证实了这种相互作用。进一步的研究表明，Ubc9 用 SUMO1 在 K159、K454 和 K626 位点使 ZHX1 发生 SUMO 化。此外，我们证明 ZHX1 的 SUMO 化调节 ZHX1 的稳定性、泛素化和转录活性。
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