Centre for Research in Biosciences, University of the West of England, Frenchay, Bristol BS16 1QY, UK.
J Chromatogr A. 2013 Jul 5;1297:37-45. doi: 10.1016/j.chroma.2013.04.006. Epub 2013 Apr 20.
The influence of pressure on the retention of several types of solute, including acids, bases and neutrals, was studied by the use of restriction capillaries added to the end of various monomeric and polymeric octadecylsilyl-modified 5μm particle size columns. Although it appeared that certain polymeric columns could give somewhat greater increases in retention with pressure, differences in behaviour between these different C18 columns were rather small. Differences in solute molecular size were most important in determining increases in retention with pressure. However, solute structure such as polarity and planarity were also influential. A prototype C30 column gave interesting selectivity changes between planar and non-planar solutes as a function of pressure. Considerable selectivity differences with pressure were shown when diverse mixtures of solutes were analysed. For the solutes studied, only minor effects of increased pressure on column efficiency and peak shape were noted.
使用限制毛细管将其添加到各种单体和聚合十八烷基硅烷修饰的 5μm 粒径色谱柱的末端，研究了压力对包括酸、碱和中性在内的几种溶质保留的影响。尽管某些聚合柱似乎可以在压力下略微增加保留时间，但这些不同 C18 柱之间的行为差异相当小。溶质分子大小的差异是决定压力下保留时间增加的最重要因素。然而，溶质结构如极性和平面性也有影响。作为压力函数的一种，原型 C30 柱在平面和非平面溶质之间表现出有趣的选择性变化。当分析不同的混合溶质时，显示出与压力有很大的选择性差异。对于研究的溶质，仅观察到压力对柱效和峰形的微小影响。
