McDowell E M, Coleman W P, De Santi A M, Newkirk C, Strum J M
Department of Pathology, University of Maryland School of Medicine, Baltimore 21201.
Anat Rec. 1990 May;227(1):111-23. doi: 10.1002/ar.1092270113.
The conducting airway epithelium of fetal Syrian golden hamsters was studied from gestational day 12 to day 15, during normal and uncontrolled diabetic pregnancies. Diabetes was induced in the pregnant hamsters by injecting streptozotocin at 60 mg/kg body weight, subcutaneously, early on gestational day 10. Cells in S-phase were labelled immunochemically with bromodeoxyuridine (BrdU), and the day on which endocrine cells and ciliated cells first appeared was determined. In control fetuses, the BrdU-labelling indices (LI's) of different anatomical airway levels were significantly different from one gestational day to the next. For example, the LI of the lobar bronchus was significantly different on each gestational day (P less than .0001), and the same was true of the bronchioles. Moreover, the difference between LI's of the lobar bronchus and bronchioles-terminal buds was highly significant on day 12 (P less than .0001), and on day 13 the differences between lobar bronchus and bronchioles, lobar bronchus and terminal buds, and bronchioles and terminal buds were also highly significant (P less than .0001). However, on gestational days 14 and 15, the LI's were reduced and were comparable at different airway levels. The BrdU-labelling indices were very consistent among fetuses of the same age, and the differences between the average LI's for pups of different litters was numerically very small. Hyperglycemia (mild, moderate, severe) did not alter LI's in the fetal airway epithelial cells. Furthermore, although glycogen was not depleted from the airway epithelium of the hyperglycemic fetuses as it was in the controls, the endocrine cells first appeared on gestational days 12, 13, and 14, respectively, in the trachea, lobar bronchus and bronchioles, followed 1 day later by the ciliated cells, in the fetuses of control and diabetic mothers. In our experimental model, induction of diabetes in the pregnant hamsters on gestational day 10 did not appear to alter development or differentiation of the fetal conducting airway epithelium.
在正常妊娠和未控制的糖尿病妊娠期间，对胎龄12至15天的叙利亚金黄地鼠胎儿的传导气道上皮进行了研究。在妊娠第10天早期，通过皮下注射链脲佐菌素（60mg/kg体重）诱导怀孕的地鼠患糖尿病。用溴脱氧尿苷（BrdU）对处于S期的细胞进行免疫化学标记，并确定内分泌细胞和纤毛细胞首次出现的日期。在对照胎儿中，不同解剖气道水平的BrdU标记指数（LI）在不同妊娠日之间存在显著差异。例如，叶支气管的LI在每个妊娠日都有显著差异（P小于0.0001），细支气管也是如此。此外，叶支气管和细支气管-终末芽的LI在第12天差异极显著（P小于0.0001），在第13天，叶支气管与细支气管、叶支气管与终末芽以及细支气管与终末芽之间的差异也极显著（P小于0.0001）。然而，在妊娠第14天和15天，LI降低，且在不同气道水平相当。同一胎龄胎儿的BrdU标记指数非常一致，不同窝幼崽平均LI之间的差异在数值上非常小。高血糖（轻度、中度、重度）并未改变胎儿气道上皮细胞的LI。此外，尽管高血糖胎儿气道上皮中的糖原不像对照组那样耗尽，但在对照和糖尿病母亲的胎儿中，内分泌细胞分别在妊娠第12天、13天和14天首次出现在气管、叶支气管和细支气管中，1天后纤毛细胞出现。在我们的实验模型中，妊娠第10天对怀孕地鼠诱导糖尿病似乎并未改变胎儿传导气道上皮的发育或分化。
