Anziani O S, Ewing S A, Barker R W
Department of Entomology, College of Agriculture, Oklahoma State University, Stillwater 74078.
Am J Vet Res. 1990 Jun;51(6):929-31.
Transstadial transmission of granulocytic Ehrlichieae in dogs was attempted using ticks, Amblyomma americanum and Dermacentor variabilis. Ticks were exposed by feeding as nymphs on acutely infected pups; adult ticks then fed to repletion on susceptible adult dogs that were monitored daily for signs of infection. Evidence of transmission was not observed in control dogs or in those exposed to D variabilis. In contrast, dogs exposed to A americanum developed serologic or clinical evidence of infection, but organisms were not seen in blood smears until corticosteroids were administered, causing recrudescence and accompanying parasitemia. At 12 days after subinoculation of blood obtained from donor adult dogs, before corticosteroids were administered, a febrile response, thrombocytopenia, and appearance of morulae in neutrophilic granulocytes were observed in 2 susceptible recipient dogs.
尝试利用美洲钝眼蜱和变异革蜱，在犬中进行粒细胞埃立克体的跨龄期传播。若蜱以急性感染幼犬为食进行暴露；然后将饱血的成年蜱喂食给易感成年犬，并每天监测其感染迹象。在对照犬或暴露于变异革蜱的犬中未观察到传播证据。相比之下，暴露于美洲钝眼蜱的犬出现了感染的血清学或临床证据，但在给予皮质类固醇导致病情复发并伴有寄生虫血症之前，在血涂片中未发现病原体。在从供体成年犬采集血液进行接种后12天，在给予皮质类固醇之前，2只易感受体犬出现发热反应、血小板减少以及嗜中性粒细胞中出现桑葚体。
