伊朗伊斯法罕慢性宫颈炎和宫颈肿瘤中扭体纤维病毒感染。

Torque teno mini virus infection in chronic cervicitis and cervical tumors in Isfahan, Iran.

机构信息

Department of Biology, Faculty of Science, Isfahan University of Medical Sciences, Isfahan, Iran.

出版信息

Intervirology. 2013;56(4):265-70. doi: 10.1159/000348514. Epub 2013 May 15.

DOI:10.1159/000348514

PMID:23689906

Abstract

OBJECTIVES

Torque teno mini virus (TTMV) is classified as the Betatorquevirus genus of Anelloviridae. Little is known about the prevalence of TTMV in humans. Worldwide, cervical cancer is the second most common cancer affecting women. This study aimed to estimate the TTMV infection frequency in cervicitis cases and cervical tumors including intraepithelial neoplasia (CIN), squamous cell carcinoma (SCC) and adenocarcinoma, and the possible role of this virus in the etiology of them in an Isfahan population.

METHODS

79 cervicitis cases and 42 tumors were collected from histopathological files of Al-Zahra Hospital in Isfahan, Iran. DNA was extracted and subjected to nested polymerase chain reaction.

RESULTS

Totally 62% of the tested samples were positive for TTMV. It was positive in 52.4% of adenocarcinoma, 68.4% of CIN and 100% SCC cases. In cervicitis, 48% of the cases were positive. In the phylogenetic construct two of the cervical tumor isolates and two of the cervicitis isolates were placed in the same cluster with already reported isolates from Japan (EF538880 and AB041962). Also, three of the cervical tumors isolated (JQ734980, JQ734981 and JQ734982) were placed in another cluster.

CONCLUSION

The presence of the virus in cervical tissues suggests possible sexual transmission of the virus.

摘要

目的

Torque teno 微小病毒（TTMV）被归类为圆环病毒科的 Betatorquevirus 属。目前人们对 TTMV 在人类中的流行情况知之甚少。在全球范围内，宫颈癌是影响女性的第二大常见癌症。本研究旨在估计宫颈炎病例和包括上皮内瘤变（CIN）、鳞状细胞癌（SCC）和腺癌在内的宫颈肿瘤中 TTMV 感染的频率，并探讨该病毒在伊朗伊斯法罕人群中对这些疾病病因学的可能作用。

方法

从伊朗伊斯法罕 Al-Zahra 医院的组织病理学档案中收集了 79 例宫颈炎病例和 42 例肿瘤。提取 DNA 并进行巢式聚合酶链反应。

结果

总共有 62%的测试样本对 TTMV 呈阳性。腺癌阳性率为 52.4%，CIN 为 68.4%，SCC 为 100%。宫颈炎病例中，有 48%的病例呈阳性。在系统发育构建中，两个宫颈肿瘤分离株和两个宫颈炎分离株与来自日本的已报道分离株（EF538880 和 AB041962）位于同一聚类中。此外，三个宫颈肿瘤分离株（JQ734980、JQ734981 和 JQ734982）被置于另一个聚类中。