Rérat A, Vaissade P, Vaugelade P
Laboratoire de Physiologie de la Nutrition, INRA, Centre de Recherches de Jouy, Jouy-en-Josas, France.
Ann Nutr Metab. 1990;34(2):119-32. doi: 10.1159/000177578.
Five pigs (mean body weight: 66.2 kg) were fitted with portal and arterial catheters and an electromagnetic flow probe around the portal vein. One week after the surgical operation, each animal was successively fed, at 3-day intervals, with two experimental meals containing 392 g dry matter either from hydrolysed or non-hydrolysed lactose together with a protein-mineral-vitamin mixture (200 g). Portal and arterial blood concentrations of glucose, galactose and amino nitrogen were measured together with the portal blood flow rate during a postprandial period of 8 h after the intake of these experimental meals. Amounts of hexoses appearing in the portal blood after hydrolysed-lactose intake were 3- and 2-fold larger within 2 and 8 h, respectively, than after non-hydrolysed-lactose intake. Thus, enzymatic hydrolysis in the intestine is the limiting factor of lactose digestion in unadapted pigs. Whatever the type of lactose ingested, glucose appeared more rapidly and in larger amounts in the portal blood than galactose. After hydrolysed-lactose intake, the amount of glucose appearing in the portal blood exceeded the amount ingested already after 5 h. This means that a fraction of galactose was transformed into glucose during the transport by the enterocyte.
选用5头猪（平均体重66.2千克），在其门静脉和动脉中植入导管，并在门静脉周围安装电磁流量探头。手术一周后，每隔3天依次给每头动物喂食两种实验餐，每种实验餐含392克干物质，分别由水解乳糖或未水解乳糖与蛋白质 - 矿物质 - 维生素混合物（200克）组成。在摄入这些实验餐后的8小时餐后期间，测量门静脉和动脉血中葡萄糖、半乳糖和氨基氮的浓度以及门静脉血流速度。摄入水解乳糖后，门静脉血中出现的己糖量在2小时和8小时内分别比摄入未水解乳糖后多3倍和2倍。因此，肠道中的酶水解是未适应的猪乳糖消化的限制因素。无论摄入何种类型的乳糖，门静脉血中葡萄糖出现得更快且量比半乳糖多。摄入水解乳糖后，门静脉血中出现的葡萄糖量在5小时后就已超过摄入量。这意味着一部分半乳糖在通过肠细胞运输过程中转化为葡萄糖。
