CBR Concepts and Experimentation Branch (Z21), Naval Surface Warfare Center, Dahlgren Division, Dahlgren, VA 22448-5162, USA.
J Appl Microbiol. 2013 Aug;115(2):398-408. doi: 10.1111/jam.12253. Epub 2013 Jun 13.
To develop test methods and evaluate survival of Bacillus anthracis Ames, B. anthracis ∆Sterne and B. thuringiensis Al Hakam spores after exposure to PES-Solid (a solid source of peracetic acid), including PES-Solid formulations with bacteriostatic surfactants.
Spores (≥ 7 logs) were dried on seven different test materials and treated with three different PES-Solid formulations (or preneutralized controls) at room temperature for 15 min. There was either no spore survival or less than 1 log (<10 spores) of spore survival in 56 of 63 test combinations (strain, formulation and substrate). Less than 2.7 logs (<180 spores) survived in the remaining seven test combinations. The highest spore survival rates were seen on water-dispersible chemical agent resistant coating (CARC-W) and Naval ship topcoat (NTC). Electron microscopy and Coulter analysis showed that all spore structures were intact after spore inactivation with PES-Solid.
Three PES-Solid formulations inactivated Bacillus spores that were dried on seven different materials.
A test method was developed to show that PES-Solid formulations effectively inactivate Bacillus spores on different materials.
开发测试方法并评估炭疽芽孢杆菌 Ames、炭疽芽孢杆菌 ∆Sterne 和苏云金芽孢杆菌 Al Hakam 孢子在暴露于 PES-Solid（过氧乙酸的固体源）后的存活情况，包括含有抑菌表面活性剂的 PES-Solid 制剂。
将（≥7 对数）孢子干燥在七种不同的测试材料上，并在室温下用三种不同的 PES-Solid 制剂（或预中和对照）处理 15 分钟。在 63 种测试组合中的 56 种（菌株、制剂和基质）中，要么没有孢子存活，要么孢子存活少于 1 对数（<10 个孢子）。在其余的七种测试组合中，存活的孢子不到 2.7 对数（<180 个孢子）。在水可分散化学剂抗性涂层（CARC-W）和海军舰艇面漆（NTC）上观察到最高的孢子存活率。电子显微镜和库尔特分析表明，在用 PES-Solid 使孢子失活后，所有孢子结构均保持完整。
三种 PES-Solid 制剂使干燥在七种不同材料上的芽孢杆菌孢子失活。
开发了一种测试方法来表明 PES-Solid 制剂可有效使不同材料上的芽孢杆菌孢子失活。
