Dr. Karin Lemmer is a scientist in the Unit Highly Pathogenic Microorganisms, Centre for Biological Threats and Special Pathogens; Professor Georg Pauli is the former Head of the Centre for Biological Threats and Special Pathogens; Sabine Howaldt is a medical technical assistant in the Unit Highly Pathogenic Microorganisms, Centre for Biological Threats and Special Pathogens; Dr. Ingeborg Schwebke is Deputy Head of the Unit Hospital Hygiene, Infection Prevention and Control, Department of Infectious Diseases; Professor Martin Mielke is Head of the Department of Infectious Diseases; and Professor Roland Grunow is Head of the Unit Highly Pathogenic Microorganisms, Centre for Biological Threats and Special Pathogens; all at the Robert Koch Institute, Berlin, Germany.
Health Secur. 2019 May/Jun;17(3):200-212. doi: 10.1089/hs.2019.0005. Epub 2019 Jun 7.
Exploratory field analyses of the inactivation capacity of disinfectants on contaminated personal protective equipment (PPE) are required to select a suitable surrogate for biohazardous agents like spores of . The objectives of our study were (1) the determination of an appropriate surrogate for the inactivation of spores of with peracetic acid (PAA), and (2) application of optimized inactivation conditions for an effective decontamination of PPE with PAA under field conditions. For inactivation studies, spores from different strains and spores were fixed by air drying on carriers prepared from PPE fabric. Time and concentration studies with PAA-based disinfectants revealed that the spores of the strain DSM 350 showed an inactivation profile comparable to that of the spores of the strain with the highest stability, implying that can serve as an appropriate surrogate. Rapid (3 to 5 minutes) and effective surface decontamination was achieved with 2% PAA/0.2% surfactant. In field studies, PPE contaminated with spores of was treated with the disinfectant. Optimizing the decontamination technique revealed that spraying in combination with brushing was effective within 5 minutes of exposure.
需要对消毒剂对污染的个人防护设备（PPE）的灭活能力进行探索性现场分析，以选择合适的替代物来替代生物危害剂，如芽孢。我们研究的目的是（1）确定一种合适的替代物，用于使用过氧乙酸（PAA）灭活芽孢，以及（2）在现场条件下，应用优化的灭活条件，对 PPE 进行有效的 PAA 消毒。在灭活研究中，来自不同菌株的芽孢和芽孢被空气干燥固定在由 PPE 织物制备的载体上。基于 PAA 的消毒剂的时间和浓度研究表明，DSM 350 菌株的芽孢表现出与稳定性最高的芽孢相似的灭活谱，这意味着可以作为合适的替代物。使用 2% PAA/0.2%表面活性剂可快速（3 至 5 分钟）和有效地对表面进行消毒。在现场研究中，用消毒剂处理污染有芽孢的 PPE。优化消毒技术表明，喷雾结合刷拭在暴露 5 分钟内有效。
