Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, Krakow 30-239, Poland.
Colloids Surf B Biointerfaces. 2013 Oct 1;110:1-7. doi: 10.1016/j.colsurfb.2013.04.014. Epub 2013 Apr 23.
In the present work, the CdSe/ZnS hydrophobic quantum dots were embedded within the polyelectrolyte nanocapsules. The core of the capsules, which consists of a mixture of the linseed oil with chloroform, was prepared using the spontaneous emulsification technique. The obtained emulsions were stabilized with lecithin and encapsulated using the layer-by-layer (LbL) adsorption of polyelectrolytes. The pair of biocompatible polyelectrolytes was used: the cationic poly-l-lysine hydrobromide (PLL) together with the anionic poly-d-glutamic acid sodium salt. The saturation LbL method, which is based on the stepwise formation of consecutive layers on the initial emulsion without the intermediate rinsing step, was applied to form the capsule shells. Their growth was evidenced by the capsule size and electrophoretic mobility measurements. The emulsion and the capsules were deposited on a mica surface and the deposit topology was examined by the means of atomic force microscopy (AFM). The presence of quantum dots within the oil cores was confirmed by recording the fluorescent spectra of the samples containing CdSe/ZnS. In order to evaluate cytotoxicity of the capsules, their influence on the viability of mouse embryonic fibroblasts was examined using the MTT test, followed by optical-microscope observation of morphology of the cells after hematoxylin-eosin staining.
在本工作中，CdSe/ZnS 疏水性量子点被嵌入到聚电解质纳米胶囊中。胶囊的核心由亚麻籽油和氯仿的混合物组成，采用自发乳化技术制备。所得乳液用卵磷脂稳定，并通过聚电解质的层层（LbL）吸附进行封装。使用了一对生物相容性聚电解质：阳离子聚-l-赖氨酸氢溴酸盐（PLL）与阴离子聚-d-谷氨酸钠盐。基于在初始乳液上不进行中间冲洗步骤而逐步形成连续层的饱和 LbL 方法，用于形成胶囊壳。通过测量胶囊的大小和电泳迁移率来证明其生长。将乳液和胶囊沉积在云母表面上，并通过原子力显微镜（AFM）检查沉积物的拓扑结构。通过记录含有 CdSe/ZnS 的样品的荧光光谱，证实了量子点在油核内的存在。为了评估胶囊的细胞毒性，通过 MTT 试验检查了它们对小鼠胚胎成纤维细胞活力的影响，然后用苏木精-伊红染色观察细胞形态的光学显微镜观察。
