Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezpominajek 8, Krakow 30-239, Poland.
Langmuir. 2010 Aug 3;26(15):12592-7. doi: 10.1021/la102061s.
The aim of this work was to develop a novel method of preparation of loaded nanosize capsules based on liquid core encapsulation by biocompatible polyelectrolyte (PE) multilayer adsorption, with or without pegylated outermost layer. Using AOT (docusate sodium salt) as emulsifier, we obtained cores, stabilized by an AOT/PLL (poly-L-lysine hydrobromide) surface complex. These positively charged cores were encapsulated by layer-by-layer adsorption of polyelectrolytes, biocompatible polyanion PGA (poly-L-glutamic acid sodium salt), and biocompatible polycation PLL. We used the saturation method for formation of consecutive layers, and we determined the optimal conditions concerning concentration of surfactant and polyelectrolytes to form stable shells. The average size of the obtained capsules was 60 nm. Pegylated external layer were prepared using PGA-g-PEG (PGA grafted by PEG poly(ethylene glycol)). The capsules were stable for at least a period of 3 months. These nanocapsules were biocompatible when tested for cytotoxicity in a cellular coculture assay and demonstrated no or very low nonspecific binding to peripheral blood mononuclear cells when tested by flow cytometry. In order to study drug effects on leukemia cells, beta-carotene and vitamin A have been encapsulated as model drugs.
这项工作的目的是开发一种新的方法，通过生物相容性聚电解质（PE）多层吸附，制备载药纳米胶囊，包括带有或不带有聚乙二醇（PEG）的最外层。使用 AOT（十二烷基硫酸钠）作为乳化剂，我们获得了由 AOT/PLL（聚-L-赖氨酸氢溴酸盐）表面复合物稳定的正电核。这些正电核通过聚电解质、生物相容性聚阴离子 PGA（聚-L-谷氨酸钠盐）和生物相容性聚阳离子 PLL 的层层吸附进行封装。我们使用饱和法形成连续层，并确定了形成稳定壳的最佳条件，涉及表面活性剂和聚电解质的浓度。所得胶囊的平均粒径为 60nm。使用 PGA-g-PEG（聚乙二醇接枝聚谷氨酸）制备了 PEG 化的外部层。这些纳米胶囊在细胞共培养测定中进行细胞毒性测试时表现出生物相容性，并且通过流式细胞术测试时对外周血单核细胞的非特异性结合非常低或没有。为了研究药物对白血病细胞的影响，β-胡萝卜素和维生素 A 已被封装为模型药物。
