Hermsen V M, Fulcher S F, Spiekerman A M, Phinizy J L, Di Tullio N W
Department of Surgery, Texas A&M University, Scott and White Hospital, Temple.
Arch Ophthalmol. 1990 Jul;108(7):1009-11. doi: 10.1001/archopht.1990.01070090111051.
The proliferation and fibrous metaplasia of retinal glial and pigment epithelial cells cause proliferative vitreoretinopathy. The immunotoxin 454A12 MAB-rRA is composed of a murine monoclonal antibody, specific for the human transferrin receptor, and is chemically linked to recombinant ricin A chain, a cellular toxin. The rapidly proliferating cells take up the immunotoxin, but non-proliferating cells do not. Using a collagen-gel medium to simulate the vitreous, we have studied the effect of the immunotoxin on fibroblast proliferation in vitro. Exposure of the fibroblasts to 1000 ng of immunotoxin per milliliter of the collagen gel medium for 10 minutes kills 96% or more of the cells for 20 days. These in vitro data indicate that the immunotoxin is effective in an environment similar to the vitreous; however, in vivo studies will be necessary to prove if it is a suitable agent for the long-term prevention of cell proliferation in the human eye.
视网膜神经胶质细胞和色素上皮细胞的增殖及纤维化生会导致增殖性玻璃体视网膜病变。免疫毒素454A12 MAB-rRA由一种针对人转铁蛋白受体的鼠单克隆抗体组成，并与细胞毒素重组蓖麻毒素A链化学连接。快速增殖的细胞会摄取免疫毒素，而非增殖细胞则不会。我们使用胶原凝胶培养基模拟玻璃体，研究了免疫毒素对体外成纤维细胞增殖的影响。将成纤维细胞暴露于每毫升胶原凝胶培养基中1000 ng免疫毒素10分钟，20天内可杀死96%或更多的细胞。这些体外数据表明，免疫毒素在类似于玻璃体的环境中是有效的；然而，需要进行体内研究以证明它是否是长期预防人眼细胞增殖的合适药物。
