Snipes K P, Hirsh D C, Kasten R W, Carpenter T E, Hird D W, McCapes R H
Department of Epidemiology and Preventive Medicine, School of Veterinary Medicine, University of California, Davis 95616.
Avian Dis. 1990 Apr-Jun;34(2):419-24.
Fifty-five serotype 3,4 isolates of Pasteurella multocida, isolated from turkeys dead from fowl cholera, were characterized (fingerprinted) genotypically for comparison with the serotype 3,4 live fowl cholera vaccine principally used in turkeys in California. Twenty-three isolates were obtained from turkeys vaccinated with the M9 live vaccine, and 32 additional isolates were from turkeys not vaccinated for fowl cholera. Methods of characterization included restriction endonuclease analysis of chromosomal DNA and ribotyping, a technique for highlighting restriction site heterogeneity of highly conserved ribosomal RNA genes and associated sequences using a radiolabeled rRNA probe. Eight different genotypes or ribotypes were detected in these isolates by the above methods. Of 23 isolates from M9-vaccinated turkeys flocks, 19 were the same ribotype as M9. Thirty of 32 isolates recovered from unvaccinated turkeys were different ribotypes from M9. The remaining two isolates resembled M9 and were recovered from two different flocks placed in succession on a turkey farm where a flock placed previously had been vaccinated with M9, suggesting interflock transmission. Ribotyping and restriction endonuclease analysis appear to be useful tools to aid in the determination of the role that the live vaccine plays in fowl cholera epidemiology.
从死于禽霍乱的火鸡中分离出55株多杀性巴氏杆菌血清型3、4菌株，对其进行基因分型（指纹图谱分析），以便与加利福尼亚州主要用于火鸡的血清型3、4禽霍乱活疫苗进行比较。23株菌株来自接种了M9活疫苗的火鸡，另外32株菌株来自未接种禽霍乱疫苗的火鸡。分型方法包括染色体DNA的限制性内切酶分析和核糖体分型，核糖体分型是一种使用放射性标记的rRNA探针来突出高度保守的核糖体RNA基因及相关序列的限制性位点异质性的技术。通过上述方法在这些菌株中检测到8种不同的基因型或核糖体分型。在来自接种M9疫苗的火鸡群的23株菌株中，19株与M9具有相同的核糖体分型。从未接种疫苗的火鸡中分离出的32株菌株中有30株与M9的核糖体分型不同。其余两株菌株与M9相似，它们是从一个火鸡场中相继饲养的两个不同鸡群中分离出来的，该火鸡场先前有一个鸡群接种了M9疫苗，这表明存在鸡群间传播。核糖体分型和限制性内切酶分析似乎是有助于确定活疫苗在禽霍乱流行病学中所起作用的有用工具。
