Daubas P, Devillers-Thiéry A, Geoffroy B, Martinez S, Bessis A, Changeux J P
U.A. CNRS D1284, Neurobiologie Moléculaire Département des Biotechnologies, Institut Pasteur, Paris, France.
Neuron. 1990 Jul;5(1):49-60. doi: 10.1016/0896-6273(90)90032-b.
In situ hybridization histochemistry reveals localized expression of the nicotinic acetylcholine receptor (nAChR) alpha 2 subunit mRNA restricted to the lateral spiriform nucleus (SpL) of the chick diencephalon. The alpha 2 nAChR transcripts are not detected in immature SpL neurons at 4.5-5 days of embryonic development. They begin to accumulate in the SpL at embryonic day 11 and increase until the newborn stage. Specific alpha 2 cDNA amplification by the polymerase chain reaction shows that during this period, the absolute content of alpha 2 mRNA increases about 20-fold. The expression of the alpha 2 nAChR gene is thus developmentally regulated and appears concomitant with the entry of cholinergic fibers into the SpL, as demonstrated by choline acetyltransferase immunohistochemistry.
原位杂交组织化学显示，烟碱型乙酰胆碱受体（nAChR）α2亚基mRNA的局部表达局限于鸡间脑的外侧螺旋状核（SpL）。在胚胎发育4.5 - 5天时，未成熟的SpL神经元中未检测到α2 nAChR转录本。它们在胚胎第11天开始在SpL中积累，并持续增加直至新生阶段。通过聚合酶链反应进行的特异性α2 cDNA扩增表明，在此期间，α2 mRNA的绝对含量增加了约20倍。因此，α2 nAChR基因的表达受到发育调控，并且如胆碱乙酰转移酶免疫组织化学所示，其出现与胆碱能纤维进入SpL同时发生。
