Daubas P, Salmon A M, Zoli M, Geoffroy B, Devillers-Thiéry A, Bessis A, Médevielle F, Changeux J P
Unité Associée D1284, Centre National de la Recherche Scientifique, Neurobiologie Moléculaire, Département des Biotechnologies, Institut Pasteur, Paris, France.
Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2237-41. doi: 10.1073/pnas.90.6.2237.
Transgenic mice carrying the complete structural gene of the alpha 2 subunit of the chicken neuronal nicotinic acetylcholine receptor (nAChR) and 7 kilobase pairs (kbp) of 5' upstream and 3 kbp of 3' downstream sequences have been generated. The transgene was stably integrated in transgenic lines and transmitted to their progeny. Avian transgene expression was predominant in the central nervous system as detected by specific alpha 2-subunit cDNA amplification. Moreover, in at least two independent mouse lines, its expression appeared to be neuron-specific and reproducibly restricted to subregions in the brain and spinal cord, as revealed by in situ hybridization histochemistry. Most cranial motor nuclei were positive, and several of the alpha 2-subunit transgene-expressing structures corresponded to cholinergic areas in rodents. This study reveals that regulatory mechanisms giving rise to neuronal-specific gene expression have been conserved at least in part between birds and mammals.
已经培育出携带鸡神经元烟碱型乙酰胆碱受体（nAChR）α2亚基完整结构基因以及5'上游7千碱基对（kbp）和3'下游3 kbp序列的转基因小鼠。转基因稳定整合在转基因品系中并传递给它们的后代。通过特异性α2亚基cDNA扩增检测到，禽类转基因表达在中枢神经系统中占主导地位。此外，原位杂交组织化学显示，在至少两个独立的小鼠品系中，其表达似乎是神经元特异性的，并且可重复地局限于脑和脊髓的亚区域。大多数颅运动神经核呈阳性，并且一些表达α2亚基转基因的结构与啮齿动物中的胆碱能区域相对应。这项研究表明，至少在鸟类和哺乳动物之间，产生神经元特异性基因表达的调控机制部分是保守的。
