铜绿假单胞菌UDP-胞壁酰三肽-D-丙氨酰-D-丙氨酸添加酶（PaMurF）的结晶及初步X射线分析
Crystallization and preliminary X-ray analysis of a UDP-MurNAc-tripeptide D-alanyl-D-alanine-adding enzyme (PaMurF) from Pseudomonas aeruginosa.
作者信息
Majce Vita, Ruane Karen M, Gobec Stanislav, Roper David I
机构信息
School of Life Sciences, University of Warwick, Gibbet Hill Road, Coventry, West Midlands CV4 7AL, England.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 May 1;69(Pt 5):503-5. doi: 10.1107/S1744309113005344. Epub 2013 Apr 30.
Abstract
The ATP-dependent UDP-MurNAc-tripeptide:D-Ala-D-Ala ligase MurF catalyses the last step in the cytoplasmic phase of peptidoglycan biosynthesis, which is critical in the formation of the bacterial cell wall and in the recycling of peptidoglycan intermediates. In this study, the crystallization of MurF from the Gram-negative pathogen Pseudomonas aeruginosa in the presence of its UDP-MurNAc-tripeptide substrate is reported. The crystals belonged to space group P212121, with unit-cell parameters a = 57.81, b = 87.29, c = 92.61 Å, and data were collected to 1.92 Å resolution, allowing study of the enzyme in the substrate-liganded form for the first time.
摘要
ATP 依赖性 UDP-N-乙酰胞壁酸-三肽：D-丙氨酰-D-丙氨酸连接酶 MurF 催化肽聚糖生物合成细胞质阶段的最后一步，这在细菌细胞壁的形成以及肽聚糖中间体的循环利用中至关重要。在本研究中，报道了来自革兰氏阴性病原体铜绿假单胞菌的 MurF 在其 UDP-N-乙酰胞壁酸-三肽底物存在下的结晶情况。晶体属于空间群 P212121，晶胞参数 a = 57.81、b = 87.29、c = 92.61 Å，数据收集至 1.92 Å 分辨率，首次使得对处于底物结合形式的该酶进行研究成为可能。
相似文献
1
Crystallization and preliminary X-ray analysis of a UDP-MurNAc-tripeptide D-alanyl-D-alanine-adding enzyme (PaMurF) from Pseudomonas aeruginosa.铜绿假单胞菌UDP-胞壁酰三肽-D-丙氨酰-D-丙氨酸添加酶（PaMurF）的结晶及初步X射线分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 May 1;69(Pt 5):503-5. doi: 10.1107/S1744309113005344. Epub 2013 Apr 30.
2
The β-lactamase gene regulator AmpR is a tetramer that recognizes and binds the D-Ala-D-Ala motif of its repressor UDP-N-acetylmuramic acid (MurNAc)-pentapeptide.β-内酰胺酶基因调节蛋白AmpR是一种四聚体，它能识别并结合其阻遏物UDP-N-乙酰胞壁酸（MurNAc）-五肽的D-Ala-D-Ala基序。
J Biol Chem. 2015 Jan 30;290(5):2630-43. doi: 10.1074/jbc.M114.618199. Epub 2014 Dec 5.
3
Phage display-derived inhibitor of the essential cell wall biosynthesis enzyme MurF.源自噬菌体展示的必需细胞壁生物合成酶MurF的抑制剂。
BMC Biochem. 2008 Dec 19;9:33. doi: 10.1186/1471-2091-9-33.
4
Pseudomonas aeruginosa MurE amide ligase: enzyme kinetics and peptide inhibitor.铜绿假单胞菌MurE酰胺连接酶：酶动力学与肽类抑制剂
Biochem J. 2009 Jun 26;421(2):263-72. doi: 10.1042/BJ20081395.
5
Crystallization and preliminary X-ray analysis of the Escherichia coli UDP-MurNAc-tripeptide D-alanyl-D-alanine-adding enzyme (MurF).大肠杆菌UDP-胞壁酰三肽-D-丙氨酰-D-丙氨酸添加酶（MurF）的结晶及初步X射线分析
Acta Crystallogr D Biol Crystallogr. 1999 Dec;55(Pt 12):2033-4. doi: 10.1107/s0907444999011786.
6
Purification, crystallization and preliminary X-ray crystallographic analysis of the UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase (MurF) from Acinetobacter baumannii.鲍曼不动杆菌UDP-N-乙酰胞壁酰三肽-D-丙氨酰-D-丙氨酸连接酶（MurF）的纯化、结晶及初步X射线晶体学分析
Acta Crystallogr F Struct Biol Commun. 2014 Jul;70(Pt 7):976-8. doi: 10.1107/S2053230X14009984. Epub 2014 Jun 19.
7
Biochemical characterization and physiological properties of Escherichia coli UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase.大肠杆菌UDP-N-乙酰胞壁酸：L-丙氨酰-γ-D-谷氨酰-内消旋二氨基庚二酸连接酶的生化特性及生理性质
J Bacteriol. 2007 Jun;189(11):3987-95. doi: 10.1128/JB.00087-07. Epub 2007 Mar 23.
8
Functional and biochemical analysis of the Chlamydia trachomatis ligase MurE.沙眼衣原体连接酶MurE的功能与生化分析
J Bacteriol. 2009 Dec;191(24):7430-5. doi: 10.1128/JB.01029-09. Epub 2009 Oct 9.
9
Crystal structures of active fully assembled substrate- and product-bound complexes of UDP-N-acetylmuramic acid:L-alanine ligase (MurC) from Haemophilus influenzae.来自流感嗜血杆菌的UDP-N-乙酰胞壁酸：L-丙氨酸连接酶（MurC）的活性完全组装的底物结合和产物结合复合物的晶体结构。
J Bacteriol. 2003 Jul;185(14):4152-62. doi: 10.1128/JB.185.14.4152-4162.2003.
10
Crystal Structure of the N-Acetylmuramic Acid α-1-Phosphate (MurNAc-α1-P) Uridylyltransferase MurU, a Minimal Sugar Nucleotidyltransferase and Potential Drug Target Enzyme in Gram-negative Pathogens.N-乙酰胞壁酸α-1-磷酸（MurNAc-α1-P）尿苷酰转移酶MurU的晶体结构，一种最小的糖核苷酸转移酶及革兰氏阴性病原菌中的潜在药物靶点酶
J Biol Chem. 2015 Apr 24;290(17):10804-13. doi: 10.1074/jbc.M114.620989. Epub 2015 Mar 12.
引用本文的文献
1
Plant peptidoglycan precursor biosynthesis: Conservation between moss chloroplasts and Gram-negative bacteria.植物肽聚糖前体生物合成：苔藓叶绿体与革兰氏阴性细菌之间的保守性。
Plant Physiol. 2022 Aug 29;190(1):165-179. doi: 10.1093/plphys/kiac176.
2
Effect of Microwave Heating on the Crystallization of Glutathione Tripeptide on Silver Nanoparticle Films.微波加热对谷胱甘肽三肽在银纳米颗粒薄膜上结晶的影响。
J Phys Chem C Nanomater Interfaces. 2017 Mar 16;121(10):5585-5593. doi: 10.1021/acs.jpcc.6b11952. Epub 2017 Feb 21.
本文引用的文献
1
Viable screening targets related to the bacterial cell wall.与细菌细胞壁相关的可行筛选靶点。
Ann N Y Acad Sci. 2013 Jan;1277:29-53. doi: 10.1111/nyas.12006. Epub 2012 Dec 26.
2
Antibiotics: a new hope.抗生素：新希望。
Chem Biol. 2012 Jan 27;19(1):3-10. doi: 10.1016/j.chembiol.2011.10.019.
3
Biochemical characterisation of the chlamydial MurF ligase, and possible sequence of the chlamydial peptidoglycan pentapeptide stem.衣原体 MurF 连接酶的生化特性，以及衣原体肽聚糖五肽茎的可能序列。
Arch Microbiol. 2012 Jun;194(6):505-12. doi: 10.1007/s00203-011-0784-8. Epub 2012 Jan 10.
4
Homology modeling and docking analyses of M. leprae Mur ligases reveals the common binding residues for structure based drug designing to eradicate leprosy.麻风分枝杆菌 Mur 连接酶的同源建模和对接分析揭示了基于结构的药物设计根除麻风病的共同结合残基。
J Mol Model. 2012 Jun;18(6):2659-72. doi: 10.1007/s00894-011-1285-z. Epub 2011 Nov 19.
5
Challenges of antibacterial discovery.抗菌药物发现的挑战。
Clin Microbiol Rev. 2011 Jan;24(1):71-109. doi: 10.1128/CMR.00030-10.
6
Bacterial cell wall assembly: still an attractive antibacterial target.细菌细胞壁组装：仍然是一个有吸引力的抗菌靶点。
Trends Biotechnol. 2011 Apr;29(4):167-73. doi: 10.1016/j.tibtech.2010.12.006. Epub 2011 Jan 12.
7
Purification and biochemical characterization of Mur ligases from Staphylococcus aureus.金黄色葡萄球菌 Mur 连接酶的纯化及生化特性研究
Biochimie. 2010 Dec;92(12):1793-800. doi: 10.1016/j.biochi.2010.07.009. Epub 2010 Jul 24.
8
Phosphorylated hydroxyethylamines as novel inhibitors of the bacterial cell wall biosynthesis enzymes MurC to MurF.磷酸羟乙胺类作为新型的细菌细胞壁生物合成酶 MurC 到 MurF 的抑制剂。
Bioorg Chem. 2009 Dec;37(6):217-22. doi: 10.1016/j.bioorg.2009.09.001. Epub 2009 Sep 10.
9
MurF inhibitors with antibacterial activity: effect on muropeptide levels.具有抗菌活性的MurF抑制剂：对胞壁肽水平的影响
Antimicrob Agents Chemother. 2009 Aug;53(8):3240-7. doi: 10.1128/AAC.00166-09. Epub 2009 May 26.
10
Mutational analysis of the substrate specificity of Escherichia coli penicillin binding protein 4.大肠杆菌青霉素结合蛋白4底物特异性的突变分析
Biochemistry. 2009 Mar 31;48(12):2675-83. doi: 10.1021/bi801993x.
Zotero 插件