Frädrich Claudia, Krausze Joern, Quade Nick, Heinz Dirk, Jahn Dieter, Härtig Elisabeth
Institute of Microbiology, University of Braunschweig, Spielmannstrasse 7, 38106 Braunschweig, Germany.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 May 1;69(Pt 5):581-4. doi: 10.1107/S1744309113010725. Epub 2013 Apr 30.
AlsR from Bacillus subtilis, a member of the LysR-type transcriptional regulator (LTTR) family, regulates the transcription of the alsSD operon encoding enzymes involved in acetoin biosynthesis. LTTRs represent the largest known family of transcriptional regulators in bacteria. In this study, AlsR82-302S100A, representing the effector domain, was produced in Escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion method in the presence of 2.1 M DL-malic acid pH 7.0 at 293 K. The crystals belonged to space group C2, with unit-cell parameters a = 142.91, b = 74.96, c = 94.39 Å, β = 110.543°. X-ray data extending to a resolution of 2.6 Å were collected.
来自枯草芽孢杆菌的AlsR是LysR型转录调节因子（LTTR）家族的成员，它调节编码参与3-羟基丁酮生物合成的酶的alsSD操纵子的转录。LTTR是细菌中已知的最大的转录调节因子家族。在本研究中，代表效应结构域的AlsR82-302S100A在大肠杆菌中产生，通过坐滴气相扩散法在293K、pH 7.0的2.1M DL-苹果酸存在下进行纯化和结晶。晶体属于空间群C2，晶胞参数a = 142.91、b = 74.96、c = 94.39 Å，β = 110.543°。收集了分辨率达2.6 Å的X射线数据。
