Experimental Biophysics Group, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.
Chemphyschem. 2001 May 18;2(5):269-72. doi: 10.1002/1439-7641(20010518)2:5<269::AID-CPHC269>3.0.CO;2-Y.
Dual-color cross-correlation spectroscopy is a special kind of fluctuation analysis which selectively probes the formation or deletion of linkages between two different fluorescently labeled molecules at extremely low concentrations. Two-photon excitation can, under certain circumstances, significantly simplify this method if different probe molecules with distinct emission properties are accessible by a common IR excitation wavelength.
双色互相关光谱学是一种特殊的波动分析,它可以在极低的浓度下选择性地探测两种不同荧光标记分子之间的形成或删除的连接。如果可以使用公共的红外激发波长来访问具有不同发射特性的不同探针分子,则双光子激发在某些情况下可以显著简化这种方法。