Institut für Physikalische und Theoretische Chemie, Technische Universität München, Lichtenbergstrasse 4, 85748 Garching, Germany.
Chemphyschem. 2001 May 18;2(5):325-8. doi: 10.1002/1439-7641(20010518)2:5<325::AID-CPHC325>3.0.CO;2-P.
Ultrafast, intra-oligomer fluorescence resonance energy transfer (FRET) between an immature green-emitting GFP-like chromophore to the mature red-emitting chromophore is found in the novel red fluorescent protein wt-DsRed (the picture shows the steady-state absorption (solid line) and emission (dotted) spectra). Since FRET is by its very nature a short range process, it represents a highly suitable method to probe oligomerization. This work describes a method preferentially applicable to the efficient screening of protein variants with mutagenetically altered surface docking sites.
在新型红色荧光蛋白 wt-DsRed 中发现了不成熟的绿色荧光蛋白样发色团与成熟的红色发色团之间超快的单体间荧光共振能量转移（FRET）（图片显示了稳态吸收（实线）和发射（点线）光谱）。由于 FRET 本质上是一种短程过程，因此它是一种非常适合探测寡聚化的方法。这项工作描述了一种优先适用于有效筛选表面对接位点发生突变的蛋白质变体的方法。
