DDR2 和 IFITM1 的共表达促进乳腺癌细胞的增殖、迁移和侵袭，抑制细胞凋亡。
Co-expression of DDR2 and IFITM1 promotes breast cancer cell proliferation, migration and invasion and inhibits apoptosis.
机构信息
Department of Cardiology, The Second Xiangya Hospital, Central South University, Changsha, 410011, Hunan, People's Republic of China.
Department of General Surgery, The Second Xiangya Hospital, Central South University, No. 139, Mid Renmin Road, Furong, Changsha, 410011, Hunan, People's Republic of China.
出版信息
J Cancer Res Clin Oncol. 2022 Dec;148(12):3385-3398. doi: 10.1007/s00432-022-04110-1. Epub 2022 Jun 28.
PURPOSE
To investigate the roles of DDR2 and IFITM1 in breast cancer (BC).
METHODS
The expression of DDR2 and IFITM1 in BC tissues and cell lines was measured. DDR2 and/or IFITM1 were knocked down in BT20 and MDA-MB-231 cells, after which the viability, mobility and apoptosis of the cells were tested. Xenograft mouse models were established through subcutaneous tumor transplantation.
RESULTS
DDR2 and IFITM1 were highly expressed in invasive BC tissues and cell lines. Overexpression of DDR2 and/or IFITM1 was associated with poorer clinical outcomes and patient survival. Knockdown of DDR2 or IFITM1 suppressed the viability and invasiveness of BT20 and MDA-MB-231 cells and restrained the growth of xenograft tumors in nude mice. Simultaneous knockdown of IFITM1 and DDR2 surpassed knockdown of IFITM1 alone in suppressing BC development.
CONCLUSIONS
DDR2 and IFITM1 are co-expressed to facilitate the malignant behaviors of BC cells and promote the development of tumors.
目的
探究 DDR2 和 IFITM1 在乳腺癌（BC）中的作用。
方法
检测 DDR2 和 IFITM1 在 BC 组织和细胞系中的表达情况。敲低 BT20 和 MDA-MB-231 细胞中的 DDR2 和/或 IFITM1，检测细胞的活力、迁移和凋亡情况。通过皮下肿瘤移植建立异种移植小鼠模型。
结果
DDR2 和 IFITM1 在浸润性 BC 组织和细胞系中高表达。DDR2 和/或 IFITM1 的过表达与较差的临床结局和患者生存相关。敲低 DDR2 或 IFITM1 抑制 BT20 和 MDA-MB-231 细胞的活力和侵袭性，并抑制裸鼠异种移植肿瘤的生长。IFITM1 和 DDR2 的同时敲低在抑制 BC 发展方面优于单独敲低 IFITM1。
结论
DDR2 和 IFITM1 共同表达促进了 BC 细胞的恶性行为，促进了肿瘤的发展。
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