Amer Said E R
Department of Zoology, Faculty of Science, Kafr El Sheikh University, Kafr El Sheikh, 33516, Egypt.
J Egypt Soc Parasitol. 2013 Apr;43(1):133-46. doi: 10.12816/0006373.
Giardiasis is a wide-spread intestinal disease of both humans and animals, with no morphological distinction between different assemblages and/or genotypes. The present study aimed to demonstrate the molecular identity of Giardia intestinalis parasites in clinical samples collected from infected cases screened at commercial laboratories and neonatal calves at dairy cattle farms, in Kafr El Sheikh. Molecular analysis based on PCR-sequence of triose phosphate isomerase (TPI) gene fragment. Results reported herein indicated that assemblage A was detected in 18/48 human isolates (37%) and 4/18 (22.2%) of the animal derived isolates, with subassemblage AII was the predominant in human samples and AI in the animal isolates. Whereas assemblage B was the predominant assemblage in the human infections (32/48: 66.7%), assemblage E was the major genotype in the animals samples (14/18: 77.8%). Genetic heterogeneity was pronounced feature in the generated sequences of all assemblages referring to wide diversity of Giardia population in Egypt. Also, results stressed the significance of neonatal calves as reservoir of zoonotic Giardia infections in human. Phylogenetic relationship of the analyzed isolates was inferred based on the reference sequences in the database.
贾第虫病是一种在人类和动物中广泛传播的肠道疾病，不同组合和/或基因型之间在形态上没有区别。本研究旨在确定从卡夫尔谢赫的商业实验室筛查的感染病例以及奶牛场新生小牛采集的临床样本中肠贾第虫寄生虫的分子特征。基于磷酸丙糖异构酶（TPI）基因片段的PCR序列进行分子分析。本文报道的结果表明，在18/48例人类分离株（37%）和4/18例动物源分离株（22.2%）中检测到A组合，其中AII亚组合在人类样本中占主导地位，AI在动物分离株中占主导地位。而B组合是人类感染中的主要组合（32/48：66.7%），E组合是动物样本中的主要基因型（14/18：77.8%）。遗传异质性是所有组合生成序列中的显著特征，这表明埃及贾第虫种群具有广泛的多样性。此外，结果强调了新生小牛作为人类人畜共患贾第虫感染储存宿主的重要性。基于数据库中的参考序列推断分析分离株的系统发育关系。
