Zhang Xiao-Xuan, Tan Qi-Dong, Zhao Guang-Hui, Ma Jian-Gang, Zheng Wen-Bin, Ni Xiao-Ting, Zhao Quan, Zhou Dong-Hui, Zhu Xing-Quan
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province, 730046, China.
College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province, 130118, China.
J Eukaryot Microbiol. 2016 Jul;63(4):498-504. doi: 10.1111/jeu.12293. Epub 2016 Feb 1.
Giardia intestinalis is a cosmopolitan protozoan parasite that can infect a range of animals, including dairy cattle. As information regarding the prevalence and genotyping of G. intestinalis infection in dairy cattle in northwestern China is limited, 2,945 feces samples from 1,224 dairy cattle in Gansu Province and from 1,614 in Ningxia Hui Autonomous Region (NXHAR) were examined between December 2012 and March 2014. The overall prevalence of G. intestinalis was 3.63% (107/2,945), with 2.63% and 4.38% in Gansu and NXHAR, respectively. Logistic regression analysis showed region, age and season to be significant risk factors for G. intestinalis infection. Assemblage analysis identified 106 assemblage E and one assemblage A at the triose phosphate isomerase (tpi) locus in this study. Intravariations were also detected at tpi, glutamate dehydrogenase (gdh) and beta giardin (bg) loci within assemblage E, showing seven, three, and five new subtypes, respectively. Moreover, 13 new multilocus genotypes (E20-E32) were observed in assemblage E. Effective strategies and measures should be taken to prevent and control giardiasis in Gansu and NXHAR.
肠道贾第虫是一种世界性的原生动物寄生虫，可感染包括奶牛在内的多种动物。由于中国西北部奶牛肠道贾第虫感染的流行情况和基因分型信息有限，于2012年12月至2014年3月期间对甘肃省1224头奶牛和宁夏回族自治区（NXHAR）1614头奶牛的2945份粪便样本进行了检测。肠道贾第虫的总体感染率为3.63%（107/2945），甘肃省和NXHAR的感染率分别为2.63%和4.38%。逻辑回归分析显示，地区、年龄和季节是肠道贾第虫感染的重要风险因素。本研究在磷酸丙糖异构酶（tpi）基因座上鉴定出106个E群和1个A群。在E群内的tpi、谷氨酸脱氢酶（gdh）和β贾第素（bg）基因座上也检测到了内部变异，分别显示出7种、3种和5种新的亚型。此外，在E群中观察到13种新的多位点基因型（E20 - E32）。应采取有效的策略和措施来预防和控制甘肃省和NXHAR的贾第虫病。
