Li Lun, Zhou Xiang-Ping, Liu Gang, Zhang Li, Ou Quan-Hong, Hao Jian-Ming
School of Physics and Electronic Information, Yunnan Normal University, Kunming 650500, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2013 Feb;33(2):340-3.
In order to investigate plant physiological process of leaf senescence and aging, Fourier transform infrared (FTIR) spectroscopy was used to study the young, mature, and old yellow leaves from seven species of evergreen trees. The spectra of the leaves from different growing period are different in the region of 1 800-700 cm(-1). The absorption ratios A1 070/A2 927, A1 070/A1 160 were used to evaluate the relative changes of polysaccharides, and A1 318/A2 922 was used to estimate the change of calcium oxalate during leaf senescence. Decomposition and curve-fitting analysis was performed in the region of 1 800 -1 500 cm(-1). The sub-band absorption ratio H1 650/H1 740 was used to evaluate the relative changes of protein in the leaves. The results show that the accumulation and mobilization of polysaccharides, protein, and calcium oxalate during leaf growing period were different in different plant species. This study demonstrates the potential of mid-infrared spectroscopy for investigation of plants senescence, as well as physiological and biochemical changes of plants.
为了研究植物叶片衰老的生理过程，利用傅里叶变换红外（FTIR）光谱对7种常绿乔木的幼叶、成熟叶和老黄叶进行了研究。不同生长时期叶片的光谱在1800 - 700 cm(-1)区域存在差异。采用吸收比A1070/A2927、A1070/A1160来评估多糖的相对变化，用A1318/A2922来估算叶片衰老过程中草酸钙的变化。在1800 - 1500 cm(-1)区域进行了解析和曲线拟合分析。利用子带吸收比H1650/H1740来评估叶片中蛋白质的相对变化。结果表明，不同植物种类在叶片生长期间多糖、蛋白质和草酸钙的积累与转运情况不同。本研究证明了中红外光谱在研究植物衰老以及植物生理生化变化方面的潜力。
