Sá e Silva M, Rissi D R, Pantin-Jackwood M, Swayne D E
Southeast Poultry Research Laboratory, Agricultural Research Service, US Department of Agriculture, 934 College Station Rd, Athens, GA 30605, USA. Email:
Vet Pathol. 2013 Nov;50(6):956-60. doi: 10.1177/0300985813490755. Epub 2013 May 22.
Infection with high-pathogenicity avian influenza virus (HPAIV) has been associated with a wide range of clinical manifestations in poultry, including severe depression in egg production and isolation of HPAIV from eggs laid by infected hens. To evaluate the pathobiology in the reproductive tract of chickens, adult hens were inoculated intranasally with 3 HPAIV strains. All 3 strains induced lesions in the reproductive tract 36 to 72 hours after inoculation. Positive immunostaining was observed in all segments of the reproductive tract, occurring predominantly in stromal cells and superficial germinal epithelium of the ovary, in mucosal epithelial cells and less often glandular epithelium throughout the oviduct, and in vascular endothelium. This study generates important data and explains previously reported virus isolation from yolk, due to ovarian virus replication, and virus recovery from albumin, due to virus replication in epithelial cells in several segments of the oviduct.
高致病性禽流感病毒（HPAIV）感染与家禽的多种临床表现有关，包括产蛋量严重下降以及从感染母鸡所产鸡蛋中分离出HPAIV。为了评估鸡生殖道中的病理生物学，成年母鸡经鼻接种了3种HPAIV毒株。接种后36至72小时，所有3种毒株均在生殖道中引发了病变。在生殖道的所有部位均观察到阳性免疫染色，主要发生在卵巢的基质细胞和表面生发上皮、整个输卵管的黏膜上皮细胞以及较少见的腺上皮细胞，以及血管内皮细胞中。这项研究产生了重要数据，并解释了先前报道的由于卵巢病毒复制而从蛋黄中分离出病毒，以及由于病毒在输卵管多个节段的上皮细胞中复制而从蛋清中回收病毒的现象。
