弗氏细胞中的核脂质。二甲基亚砜诱导红细胞分化过程中甘油二酯的变化图谱。

Nuclear lipids in Friend cells. Shifted profile of diacylglycerol during erythroid differentiation induced by DMSO.

作者信息

Trubiani O, Martelli A M, Manzoli L, Santavenere E, Cocco L

机构信息

Institute of Cytomorphology CNR, University of Chieti, Italy.

出版信息

Cell Biol Int Rep. 1990 Jun;14(6):559-66. doi: 10.1016/0309-1651(90)91183-5.

DOI:10.1016/0309-1651(90)91183-5

PMID:2369764

Abstract

Friend cells were labelled for 90 min. with [3H]-Glycerol and the radioactivity in DAG# and TG was measured. The relative percentage of radiolabelled DAG differs in isolated nuclei as compared to intact cells. Moreover the level of newly synthesized DAG decreases in nuclei isolated from cells treated with DMSO for 24 hrs. as well as in nuclei from cells treated for 96 hrs. and terminally differentiated in the erythroid pathway. Since these changes are not seen in intact cells, the results are consistent with the hypothesis that nuclear lipids and namely the products of polyphosphoinositide hydrolysis, such as DAG, are involved in the regulation of the processes leading to cell differentiation.

摘要

用[³H] - 甘油标记Friend细胞90分钟，然后测量二酰甘油（DAG）#和甘油三酯（TG）中的放射性。与完整细胞相比，分离细胞核中放射性标记的DAG的相对百分比有所不同。此外，在用二甲基亚砜（DMSO）处理24小时的细胞分离出的细胞核中，以及在用二甲基亚砜处理96小时并在红系途径中终末分化的细胞的细胞核中，新合成的DAG水平均下降。由于在完整细胞中未观察到这些变化，这些结果与以下假设一致，即核脂质，特别是多磷酸肌醇水解产物，如DAG，参与导致细胞分化过程的调节。