Tsiftsoglou A S, Sartorelli A C
Cancer Res. 1979 Oct;39(10):4058-63.
Friend erythroleukemia cells grown in culture and induced to differentiate along the erythroid developmental pathway by dimethyl sulfoxide (DMSO) were used as a model system to investigate the requirement for cellular replication to express a differentiated erythroid phenotype. That cytokinesis is not essential for DMSO-induced erythroid differentiation as measured by the synthesis and accumulation of hemoglobin was shown by experiments using cytochalasin B. In these studies, hemoglobin was found to accumulate in Friend cells treated simultaneously with DMSO and cytochalasin B; such treatment caused cells to become enlarged and multinucleated due to inhibition of cytokinesis by cytochalasin B. In contrast, exposure of cells to cytochalasin B for at least 48 hr prior to DMSO caused significant inhibition of erythroid differentiation. The findings support the concept that cellular division and, thereby the production of new cellular types are not required for gene activation and the expression of an erythroid phenotype. These effects of cytochalasin B on DMSO-induced differentiation of Friend leukemia cells also suggest plasma membrane-cytoskeleton involvement in the initiation of the erythroid maturation process in this system.
在培养中生长并通过二甲基亚砜（DMSO）诱导沿红系发育途径分化的Friend红白血病细胞被用作模型系统，以研究细胞复制对表达分化红系表型的必要性。使用细胞松弛素B的实验表明，通过血红蛋白的合成和积累来衡量，胞质分裂对于DMSO诱导的红系分化并非必不可少。在这些研究中，发现血红蛋白在同时用DMSO和细胞松弛素B处理的Friend细胞中积累；这种处理由于细胞松弛素B对胞质分裂的抑制作用而导致细胞变大并多核化。相反，在DMSO处理之前将细胞暴露于细胞松弛素B至少48小时会导致红系分化受到显著抑制。这些发现支持了这样一种概念，即细胞分裂以及由此产生新的细胞类型对于基因激活和红系表型的表达并非必需。细胞松弛素B对DMSO诱导的Friend白血病细胞分化的这些作用还表明，质膜-细胞骨架参与了该系统中红系成熟过程的起始。
