通过组合两种 amiR 或靶向高度保守的病毒基因组区域来提高人工 microRNA（amiR）介导的对芜菁花叶病毒抗性的有效性。
Improving the effectiveness of artificial microRNA (amiR)-mediated resistance against Turnip mosaic virus by combining two amiRs or by targeting highly conserved viral genomic regions.
机构信息
Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València, València, Spain.
出版信息
J Virol. 2013 Jul;87(14):8254-6. doi: 10.1128/JVI.00914-13. Epub 2013 May 22.
Abstract
A drawback of recent antiviral therapies based on the transgenic expression of artificial microRNAs (amiRs) is the ease with which viruses generate escape mutations. Here, we show two alternative strategies for improving the effectiveness of resistance in plants. First, we expressed two amiRs complementary to independent targets in the viral genome, and second, we designed amiRs complementary to highly conserved RNA motifs in the viral genome.
摘要
基于人工 microRNA（amiR）转基因表达的抗病毒疗法的一个缺点是病毒很容易产生逃逸突变。在这里，我们展示了两种改进植物抗性效果的替代策略。首先，我们表达了两个与病毒基因组中独立靶标互补的 amiR，其次，我们设计了与病毒基因组中高度保守的 RNA 基序互补的 amiR。
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