Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
Jpn J Infect Dis. 2013;66(3):180-8. doi: 10.7883/yoken.66.180.
Salmonella Typhimurium is an important nontyphoidal Salmonella serovar associated with foodborne diseases in many parts of the world. This organism is the major causative agent of nontyphoidal salmonellosis in Malaysia. We aimed to investigate the genetic profiles of the strains isolated from clinical, zoonotic, and dietary sources in Malaysia using multilocus variable number tandem repeat analysis (MLVA) and pulsed-field gel electrophoresis (PFGE). By focusing on the 5 common variable number tandem repeat (VNTR) loci, we found that PFGE (D = 0.99) was more discriminative than MLVA (D = 0.76). The low MLVA score might be because of a lack of VNTR loci STTR6 (81.0%) and STTR10pl (76.2%). Both subtyping methods suggested that our S. Typhimurium strains were largely endemic with limited genetic variation. Furthermore, we observed that biphasic S. Typhimurium strains were dominant (99%) and multidrug resistance was prevalent (50%) within our sample pool. The most frequently observed phenotypes were resistance to compound sulfonamides (49%), tetracycline (51%), and streptomycin (52%). In this study, we documented the genetic relationship, antimicrobial resistance characteristics, and flagellar-phase dominance among S. Typhimurium strains found in Malaysia.
鼠伤寒沙门氏菌是一种重要的非伤寒血清型沙门氏菌，与世界许多地区的食源性疾病有关。该病原体是马来西亚非伤寒性沙门氏菌病的主要病原体。我们旨在使用多位点可变数目串联重复分析（MLVA）和脉冲场凝胶电泳（PFGE）研究从临床、人畜共患病和饮食来源分离的菌株的遗传特征。通过关注 5 个常见的可变数目串联重复（VNTR）基因座，我们发现 PFGE（D=0.99）比 MLVA（D=0.76）更具区分力。低 MLVA 评分可能是因为缺乏 VNTR 基因座 STTR6（81.0%）和 STTR10pl（76.2%）。这两种分型方法均表明，我们的鼠伤寒沙门氏菌菌株主要是地方性的，遗传变异有限。此外，我们观察到我们的样本池中存在大量二相性鼠伤寒沙门氏菌菌株（99%）和普遍存在的多药耐药性（50%）。最常见的表型是对复方磺胺类药物（49%）、四环素（51%）和链霉素（52%）的耐药性。在本研究中，我们记录了在马来西亚发现的鼠伤寒沙门氏菌菌株的遗传关系、抗菌药物耐药特征和鞭毛相优势。
