Zakaria Zunita, Hassan Latiffah, Sharif Zawiyah, Ahmad Norazah, Mohd Ali Rohaya, Amir Husin Suraya, Mohamed Sohaimi Norfitriah, Abu Bakar Shafini, Garba Bashiru
Institute of Bioscience, Universiti Putra Malaysia, Serdang 43400, Malaysia.
Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang 43400, Malaysia.
Animals (Basel). 2022 Jan 1;12(1):97. doi: 10.3390/ani12010097.
This study was undertaken to determine the virulence, antimicrobial resistance and molecular subtypes of in the Central Region of Peninsular Malaysia. A total of 45 Enteritidis were detected from live chicken (cloacal swab), and chicken products (fresh and ready-to-eat meat) samples upon cultural isolation and serotyping. Similarly, an antimicrobial susceptibility test based on the Kirby Bauer disk diffusion method as well as antimicrobial resistance AMR genes, virulence determinants and multilocus sequence typing (MLST) typing were conducted after the Whole Genome Sequencing and analysis of the isolates. The results indicate that sequence types ST1925 (63.7%), and ST11 (26.5%) were the predominant out of the seven sequence types identified (ST292, ST329, ST365, ST423 and ST2132). The phenotypic antimicrobial profile corresponds to the genotypic characterization in that the majority of the isolates that exhibited tetracycline, gentamycin and aminoglycoside resistance; they also possessed the and β-Lactam resistance genes. However, isolates from cloacal swabs showed the highest number of resistance genes compared to the chicken products (fresh and ready-to-eat meat) samples. Furthermore, most of the virulence genes were found to cluster in the pathogenicity island (SPI). In this study, all the isolates were found to possess SPI-1, which codes for the type III secretion system, which functions as actin-binding proteins (SptP and SopE). The virulence plasmid (VP) genes (, ) were present in all genotypes except ST365. The findings of this study, particularly with regard to the molecular subtypes and AMR profiles of the Enteritidis serotype shows multidrug-resistance features as well as genetic characteristics indicative of high pathogenicity.
本研究旨在确定马来西亚半岛中部地区肠炎沙门氏菌的毒力、抗菌药物耐药性和分子亚型。通过培养分离和血清分型，从活鸡（泄殖腔拭子）以及鸡肉产品（新鲜和即食肉）样本中总共检测到45株肠炎沙门氏菌。同样，在对分离株进行全基因组测序和分析后，基于 Kirby Bauer 纸片扩散法进行了抗菌药物敏感性试验，以及抗菌药物耐药性（AMR）基因、毒力决定因素和多位点序列分型（MLST）分型。结果表明，在鉴定出的七种序列类型（ST292、ST329、ST365、ST423 和 ST2132）中，序列类型 ST1925（63.7%）和 ST11（26.5%）占主导地位。表型抗菌谱与基因型特征相对应，即大多数表现出对四环素、庆大霉素和氨基糖苷类耐药的分离株；它们还拥有β-内酰胺耐药基因blaTEM和blaSHV。然而，与鸡肉产品（新鲜和即食肉）样本相比，泄殖腔拭子分离株显示出的耐药基因数量最多。此外，大多数毒力基因被发现聚集在沙门氏菌致病岛（SPI）中。在本研究中，发现所有分离株都拥有SPI-1，其编码III型分泌系统，该系统作为肌动蛋白结合蛋白（SptP和SopE）发挥作用。除ST365外，所有基因型中均存在毒力质粒（VP）基因（spvC和spvR）。本研究的结果，特别是关于肠炎沙门氏菌血清型的分子亚型和AMR谱，显示出多药耐药特征以及表明高致病性的遗传特征。
