Stack T, Reeds P, Preston T, Hay S, Lloyd D J, Aggett P J
Department of Child Health, Foresterhill, Aberdeen, UK.
Eur J Clin Nutr. 1990 Mar;44(3):231-4.
Hydrolysed yeast protein labelled with 15N was used to measure protein turnover, protein synthesis and protein breakdown in preterm infants. The yeast tracer was given as a bolus intragastric dose and protein turnover was determined from the 15N enrichment of urinary ammonia over known periods of about 12 h. Six boys (birthweight less than 1500 g) with gestation of 27-35 weeks were studied either two or three times at post-natal ages ranging from 13 to 54 d. There was no significant correlation between protein turnover with increasing post-conceptional or post-natal age. There was considerable interindividual variation and reproducibility varied between different infants. We suggest that this is a convenient non-invasive technique for monitoring serially nitrogen and protein metabolism in infants and that as such it merits further assessment.
用15N标记的水解酵母蛋白来测量早产儿的蛋白质周转、蛋白质合成和蛋白质分解。酵母示踪剂通过胃内推注给药，蛋白质周转通过在约12小时的已知时间段内尿氨的15N富集来确定。对6名出生体重低于1500克、孕龄为27 - 35周的男婴在出生后13至54天进行了两到三次研究。蛋白质周转与孕龄或出生后年龄的增加之间没有显著相关性。个体间存在相当大的差异，不同婴儿之间的可重复性也有所不同。我们认为这是一种用于连续监测婴儿氮和蛋白质代谢的方便的非侵入性技术，因此值得进一步评估。
